|
|
|
|
|
|
| Research on Fluorescence Spectral Characteristics of Phycocyanin under Different Habitat Conditions |
| ZHANG Xiao-ling1,2,3, YIN Gao-fang1,2,3, ZHAO Nan-jing1,2,3*, QIN Zhi-song1,2,3, XIAO Xue1,2,3, DUAN Jing-bo1,2,3, YANG Rui-fang1,2,3, TU Meng-di1,2,3, LIU Jian-guo1,2,3, LIU Wen-qing1,2,3 |
1. Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China
2. University of Science and Technology of China, Hefei 230036, China
3. Key Laboratory of Optical Monitoring Technology for Environment, Anhui Province, Hefei 230031, China |
|
|
|
|
Abstract The development of cyanobacterial biomass detection technology is an important part to deal with the frequent occurrence of water bloom events. Phycocyanin, to some extent, as a special protein of cyanobacteria, is more accurate reaction in natural water cyanobacterial biomassthan chlorophyll, thus become an important index in cyanobacterial biomass detection. This paper, with different light intensity and different growth stages as the research objects. compares the reliability of the two methods of the envelope method and the single point method , exploring the fluorescence spectral characteristics of phycocyanin in vivo Microcystis Anabaena by using three-dimensional fluorescence spectroscopy. The results show that：(1) The fluorescence intensity increases with the growth of long-term; (2) It is more reliable to use envelope method to analyze the characteristic fluorescence spectra of the fluorescent spectrum than the single point method. (3) The fluorescence spectra of Phycocyanin in vivo Microcystis were basically unchanged in EX=614 nm/ EM=654 nm. The excitation wavelength of the phycocyanin fluorescence in vivo Microcystis decreases with the growth with 610 and 620 nm in the long term, and the emission wavelength varied between 650 and 660 nm, which is related to the sample particle size and spectral scanning mode. This study provided the experimental basis for the development of the fluorescent detection technology of cyanobacteria.
|
|
Received: 2015-10-16
Accepted: 2016-04-22
|
|
|
|
Corresponding Authors:
ZHAO Nan-jing
E-mail: xlzhang@aiofm.ac.cn
|
|
[1] Beutler M, Wiltshire K H, Meyer B, et al. Photosynthesis Research, 2002, (72): 39.
[2] Han Boping, Han Zhiguo, FU Xiang. Algal Photosynthesis: Mechanisms and Models. Beijing:Science Press, 2003. 6.
[3] Su Rongguo, Hu Xupeng, Zhang Chuansong, et al. Journal of Tropical Oceanography, 2008, 27(5): 24.
[4] McQuaid N, Zamyadi A, Prevost M, et al. Journal of Environmental Monitoring, 2011, (13): 455.
[5] Yin Gaofang, Zhao Nanjing, et al. Acta Optica Sinica, 2014, 34(9): 09300051.
[6] Wang Zhigang, Liu Wenqing, Zhang Yujun, et al. China Environmental Science, 2008, 28(2): 136.
[7] Wang Yong, Qian Kaixian. Prog. Biochem. Biophys., 1999, 26(5): 457.
[8] Larisa Poryvkina, Sergey Babichenko, Aina Leeben. Proceedings of EARSeL-SIG-Workshop LIDAR, 2000, 6: 16.
[9] Bastien C1, Cardin R, Veilleux E, et al. Journal of Environmental Monitoring, 2011, 13(1): 110.
[10] Zeep R G, Sheldon W M, Moran, et al. Marine Chemistry, 2004, 89(4): 15. |
| [1] |
WANG Xiang1, 2, YIN Gao-fang1*, ZHAO Nan-jing1, GAN Ting-ting1, YANG Rui-fang1, QIN Zhi-song3, DONG Ming1, 2, CHEN Min1, 2, DING Zhi-chao1, 2, QI Pei-long1, 2, WANG Lu1, 2, MA Ming-jun1, 2, MENG De-shuo1, LIU Jian-guo1. Fast Measurement of Primary Productivity in the Yellow Sea and Bohai Sea Based on Fluorescence Kinetics Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(03): 990-996. |
| [2] |
ZHANG Fa-yu1, YU Jin-wei1, ZHANG Liu2, SHENG Jing-meng1, YUAN Meng-yuan1, LU Yi-nan1, WANG Jia-quan1* . UV-Vis Spectrum Characteristics of Phycocyanin Purification in Water from Chao Lake [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(03): 806-810. |
| [3] |
LI Yao1, 2, ZHANG Li-fu1*, HUANG Chang-ping1, WANG Jin-nian1, CEN Yi1. Monitor of Cyanobacteria Bloom in Lake Taihu from 2001 to 2013 Based on MODIS Temporal Spectral Data[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(05): 1406-1411. |
| [4] |
ZHANG Jing, WEI Yu-chun*, WANG Guo-xiang, CHENG Chun-mei, XIA Xiao-rui . UV-Vis Spectrum Characteristics of Phycocyanin in Water from Taihu Lake [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(05): 1297-1301. |
| [5] |
LIU Jing1，2, LIU Wen-qing2, ZHAO Nan-jing2*, ZHANG Yu-jun2, MA Ming-jun2, YIN Gao-fang2, DAI Pang-da2, WANG Zhi-gang2, WANG Chun-long2, DUAN Jing-bo2, YU Xiao-ya2, FANG Li2 . The Method of Phytoplankton Photosynthesis Activity In-Situ Measurement Based on Light Induced Fluorescence[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(09): 2443-2447. |
| [6] |
ZHOU Wen, SUN Zhao-hua, CAO Wen-xi, WANG Gui-fen . Variations in the Optical Absorption and Attenuation Properties of Cultured Phytoplankton and Their Relationships with Cell Size[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(12): 3347-3352. |
| [7] |
ZHANG Shan-shan1, SU Rong-guo1*, DUAN Ya-li1, SONG Zhi-jie2, WANG Xiu-lin1 . Research on the 3D Discrete Fluorescence Spectrum Technique for Differentiation of Phytoplankton Population[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(03): 732-736. |
| [8] |
SONG Yu1，2, SONG Xiao-dong1, GUO Qing-hai1, TANG Li-na1* . Remote Sensing Monitoring and Pre-Alarming of Algal Blooms in Taihu Lake [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(03): 753-757. |
| [9] |
ZHAO Zhi-min1，2, HONG Xiao-qin1, LI Peng1，JIN Xiao-dong1 . Spectral Analysis of Cyanobacteria Chlorophyll in Polluted Wate [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(06): 1596-1599. |
| [10] |
SHAO Si-mi1, SU Hai-nan1, ZHANG Xi-ying1*, PENG Guo-hong2, ZHOU Bai-cheng1, ZHANG Yu-zhong1. The Active Conformation of Allophycocyanin from Spirulina platensis Studied with Spectroscopic Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(06): 1643-1646. |
| [11] |
LIU Bao1,4, SU Rong-guo1*, SONG Zhi-jie2, ZHANG Fang3，WANG Xiu-lin1 . Research on the 3D Fluorescence Spectra Differentiation of Phytoplankton by Coiflet2 Wavelet [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(05): 1275-1278. |
| [12] |
ZHANG Ting-lu, DU Xiang-zhi, XU Qing-na, QIU Guo-qiang. Application of 1D Wavelet Analysis in Detecting Species of Harmful Algae Blooms with Absorption Spectra of Phytoplankton [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(10): 2743-2747. |
| [13] |
YAN Shi-gan1,3,CHEN Xiu-lan1,ZHANG Xi-ying1,ZHOU Bai-cheng1,2,ZHANG Yu-zhong1*. Spectral Changes of C-Phycocyanin with Different Molar Ratios of SPDP[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(05): 1115-1117. |
| [14] |
LU Lu1,SU Rong-guo1,2,WANG Xiu-lin1,2*,ZHU Chen-jian1,2. Study on the Characters of Phytoplankton Chlorophyll Fluorescence Excitation Spectra Based on Fourth-Derivative[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(11): 2307-2312. |
| [15] |
TANG Xiao-jing1,ZHANG Qian-qian1*,LEI Shu-he2,SU Rong-guo1,ZHU Chen-jian1,WANG Xiu-lin1 . Research on Characterization Analysis of Synchronous Fluorescence Spectra of Living Phytoplankton[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(03): 556-559. |
|
|
|
|
