| 光谱学与光谱分析 |
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| Study on the Characters of Phytoplankton Chlorophyll Fluorescence Excitation Spectra Based on Fourth-Derivative |
| LU Lu1,SU Rong-guo1,2,WANG Xiu-lin1,2*,ZHU Chen-jian1,2 |
1. Marine Polluted Eco Chemistry Lab, Ocean University of China, Qingdao 266003, China
2. Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266003, China |
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Abstract Chlorophyll fluorescence excitation spectra of six phytoplankton species, belonging to Bacillariophyta and Dinophyta, were dealt by fourth-derivative analysis with the Matlab program. The results show that between 350 nm and 550 nm six fluorescence peaks were found in the fourth-derivative spectra, which are representatives of non-pigments, chlorophylls and carotenoides respectively. The method makes Bacillariophyta and Dinophyta more distinguishable when the fourth-derivative spectra are compared with the chlorophyll fluorescence excitation spectra. It can be used not only to discriminate the two groups of algaes, but also to reduce the effect of noise. The fluorescence peaks in the fourth-derivative spectra are proved to be stable.
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Received: 2006-09-02
Accepted: 2006-12-08
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Corresponding Authors:
WANG Xiu-lin
E-mail: xlwang@ouc.edu.cn
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| Cite this article: |
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LU Lu,SU Rong-guo,WANG Xiu-lin, et al. Study on the Characters of Phytoplankton Chlorophyll Fluorescence Excitation Spectra Based on Fourth-Derivative[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(11): 2307-2312.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2007/V27/I11/2307 |
[1] YE Shu-feng, HUANG Xiu-qing(叶属峰，黄秀清). Marine Environmental Science(海洋环境科学), 2003, 22(2): 10.
[2] GAO Hong-feng, JIAO Nian-zhi(高洪峰，焦念志). MarineScience(海洋科学), 1997, (3): 51.
[3] ZHANG Qian-qian, LEI Shu-he, WANG Xiu-lin, et al(张前前，类淑和，王修林，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(10): 1227.
[4] Lutz V A, Sathyendranath S. J. Phycol., 1998, 34: 214.
[5] Schreiber U. Chlorophyll Fluorescence and Photosynthetic Energy Conversion: Simple Introductory Experiments with the Teaching-pam Chlorophyll Fluorometer. Germany：Heinz Walz GmbH, 1997. 5.
[6] Talsky G. Derivative Spectrophotometry: Low and High Order. VCH: Weinheim, 1994. 10.
[7] FAN Hu, SHENG Liang-quan, TONG Hong-wu, et al(樊 虎，盛良全，童红武，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(10): 1627.
[8] Morrey, J. R. Anal. Chem.，1968, 40: 905.
[9] Mille D F, Schofield O M, et al. Limmology Oceanography, 1997, 42(5): 1240.
[10] Aguirre-Gómez R, Weeks A. R, et al. International Journal of Remote Sensing, 2001, 22: 315.
[11] Hoepffner N, Sathyendranath S. Mar. Ecol. Prog. Ser.，1991, 73: 11.
[12] Hoepffner N, Sathyeodranath S. J. of Geophysical Res., 1993, 98: 22789.
[13] Poryvkina L, Babichenko S, Proceeding of EARSeL-SIG-Workshop LIDAR, Dresden/FRG, 2000, (1): 224.
[14] Yentsch C S, Yentsch C M. J. Mar. Res.，1979, 37: 471.
[15] Johnsen G. Nelson N B, et al. Mar. Ecol. Prog. Ser.，1994, 114: 245. |
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