光谱学与光谱分析 |
|
|
|
|
|
Research on Molecular Spectra of Interactions Between Salvianolic Acid A and Salvianolic Acid B with Insulin, and Effect of Glucose on the Binding |
YANG Wen-yue1, CUI Lin1, QU Ling-ling1, XU Ning1, HUANG Yun1,2*, CUI Li-jian3*, ZHAN Wen-hong1, ZHAO Ding1 |
1. Pharmaceutical College, Hebei Medical University, Shijiazhuang 050017, China 2. Institute of Chinese Integrative Medicine, Hebei Medical University, Shijiazhuang 050017, China3. Experimental Center, Hebei University of Chinese Medicine, Shijiazhuang 050091, China |
|
|
Abstract The interactions of Salvianolic acid A (SAA) and Salvianolic acid B (SAB) with insulin were studied by using fluorescence spectroscopy, UV-vis spectroscopy and ATR-FTIR spectroscopy in simulating physiological condition (pH 7.40). The fluorescence quenching of insulin by SAA and SAB were static quenching process. The results of synchronous fluorescence and three-dimensional fluorescence spectra suggested no obvious conformation changes of insulin after SAA or SAB binding. But ATR-FTIR spectra showed that SAA and SAB could change the secondary structures of insulin, of which β-turns decreased and random coil increased accompanied with α-helices and β-sheets no clear change. The glucose might influenced the the bioactivity of insulin in the SAA-insulin and SAB-insulin systems by changing the binding constants of SAA (or SAB) with insulin and exacerbating the changes of insulin conformation and relative contents of α-helices.
|
Received: 2015-05-06
Accepted: 2015-09-17
|
|
Corresponding Authors:
HUANG Yun, CUI Li-jian
E-mail: huangyun9317@126.com; cuilijianzy@126.com
|
|
[1] Zhang Mei, Li Xu, Qiu Genquan, et al. Jorunal of Chinese Medicinal Materials,2005, 28(6): 529. [2] Rong Xiuhua, Han Xuewen. Jorunal of Binzhou Medical College,1996, 19(6): 539. [3] Zhao Ling. Journal of Zhejiang College of Traditional Chinese Medicine,2009, 33(1): 82. [4] Wang Xiaomei, Zhen Zhuoli, Chen Xiaofen, et al. Journal of Hebei Medicine,2005, 11(9): 769. [5] Hye Sook Kang, Hae Young Chung, Dae Seok Byun, et al. Arehievs of Pharmacal Researeh,2003, 26(1): 24. [6] Fan Huaying, Fu Fenghua, Yang Mingyan, et al. Thrombosis Research,2010, 126(1): 17. [7] Huang Z S, Zeng C L, Zhu L J, et al. Journal of Thrombosis and Haemostasis,2010, 8(6): 1383. [8] Wang Shoubao, Tian Shuo, Fan Yang, et al. European Journal of Pharmacology,2009, 615(1-3): 125. [9] Guo Yongxue, Xiu Zhilong, Zhang Daijia, et al. Joumal of Pharmaceutical and Biomedical Analysis,2007, 43(4): 1249. [10] Yang Yanyi, Tian Ying, Tian Yanjiao, et al. Chinese Journal of Geriatric Care,2010, 8(4): 22. [11] Ye Yunhua. University Chemistry,2010, 25: 19. [12] Pullen R A, Lindsay D G, Stephen P Wood, et al. Nature, 1976, 259(5542): 369. [13] Kazarian S G, Chan K L A. Biochimica Et Biophysica Acta-Biomembranes,2006, 1758(7):858. [14] Hua Shi, Ling Xiong, Yang Kunyun, et al. Journal of Molecular Structure,1998, 446: 137. [15] Cui Fengling, Qin Lixia, Zhang Guisheng, et al. Journal of Pharmaceutical and Biomedical Analysis,2008, 48: 1029. [16] Li Qiang, Yang Wenyue, Qu Lingling, et al. Journal of Spectroscopy,2014, 834501:7. [17] Huang Yun, Cui Lijian, Wang Jianming, et al. Journal of Luminescence,2012, 132: 357. [18] Yang Jie, Qu Lingling, Yang Wenyue, et al. Journal of Spectroscopy,2014, 386586:9. [19] Huang Yun, Cui Lijian, Chen Chen, et al. Chinese Pharmacological Bulletin,2010, 26(6): 754. [20] Cui Fengling, Qin Lixia, Hu Xing, et al. Journal of Pharmaceutical and Biomedical Analysis,2008, 48: 1029. [21] Huang Yun, Cui Lijian, Wang Jianming, et al. European Journal of Medicinal Chemistry,2011, 46: 6039. [22] Zhang Xuan, Huang Lixin, Nie Songqing, et al. Journal of Chinese Pharmaceutical Sciences,2003, 12(1): 11. |
[1] |
WANG Fei1,2, LI Huan1, YANG Ke1, TEULET Philippe2, CRESSAULT Yann2. Computation of Equilibrium Compositions of GMAW Arc Plasmas and Its Applications in Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 1998-2003. |
[2] |
XU Chen1, 2, HUA Xue-ming1, 2*, YE Ding-jian1, 2, MA Xiao-li1, 2, LI Fang1, 2, HUANG Ye1, 2. Study of the Effect of Interference during Multi-Wire GMAW Based on Spectral Diagnosis Technique[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 1993-1997. |
[3] |
NIE Mei-tong1,2, XU De-gang1,2*, WANG Yu-ye1,2*, TANG Long-huang1,2, HE Yi-xin1,2, LIU Hong-xiang1,2, YAO Jian-quan1,2. Investigation on Characteristics of Edible Oil Spectra with Terahertz Time-Domain Attenuated Total Reflection Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2016-2020. |
[4] |
LI Zheng-hui1,3, YAO Shun-chun1,3*, LU Wei-ye2, ZHU Xiao-rui1,3, ZOU Li-chang1,3, LI Yue-sheng2, LU Zhi-min1,3. Study on Temperature Correction Method of CO2 Measurement by TDLAS[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2048-2053. |
[5] |
HU Wen-bin1, MA Zhi-min1*, TIAN Meng1, ZHAO Xiao-hong1, HU Xiang-yang2. Multispectral-Based Particle Image Velocimetry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2038-2043. |
[6] |
LIU Lu-yao1, ZHANG Bing-jian1,2*, YANG Hong3, ZHANG Qiong3. The Analysis of the Colored Paintings from the Yanxi Hall in the Forbidden City[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2054-2063. |
[7] |
LI Xiao-jun, HE Xian-li, SONG Rui-juan. Theoretical Study of Structures, Stabilities, and Infrared Spectra of the Alkali-Metal (Li2F)nM (M=Li, Na, K; n=1, 2) Clusters[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2064-2069. |
[8] |
JIAN Kuo1,2,LIU Shun-xi4,CHEN Yi-lin3,FU Xue-hai2,3*. Infrared Spectroscopic Study on the Structure Evolution of Low Rank Coal and Its Correlation with Carbon Isotope of Alkane Gas in Pyrolysis Process[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2070-2075. |
[9] |
WANG Shuai1, XU Jun-ping1, WANG Nan1, LEI Wan-ying1, FAN Xi-yan1, DOU Sen2, 3*. Structural Characteristics of Mineral-Microbial Residues Formed by Microbial Utilization of Lignin Joined with Fe, Al, Mn-Oxides Based on FT-IR and SEM Techniques[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2086-2093. |
[10] |
WANG Wen-xiu, PENG Yan-kun*, FANG Xiao-qian, BU Xiao-pu. Characteristic Variables Optimization for TVB-N in Pork Based on Two-Dimensional Correlation Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2094-2100. |
[11] |
HU Hua-ling1, 2, 3, LI Meng2, 3*, HE Xiao-song2, 3, XI Bei-dou2, 3, ZHANG Hui2, 3, LI Dan2, 3, HUANG Cai-hong2, 3, TAN Wen-bing2, 3. FTIR Spectral Characteristics of Rice Plant Growing in Mercury Contaminated Soil[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2081-2085. |
[12] |
LE Ba Tuan1, 3, XIAO Dong1*, MAO Ya-chun2, SONG Liang2, HE Da-kuo1, LIU Shan-jun2. Coal Classification Based on Visible, Near-Infrared Spectroscopy and CNN-ELM Algorithm[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2107-2112. |
[13] |
WANG Dong, LIU Shan-jun*, MAO Ya-chun, WANG Yue, LI Tian-zi. A Method Based on Thermal Infrared Spectrum for Analysis of SiO2 Content in Anshan-Type Iron[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2101-2106. |
[14] |
MA Dian-xu1, LIU Gang1*, OU Quan-hong1, YU Hai-chao1, LI Hui-mei1, SHI You-ming2. Discrimination of Common Wild Mushrooms by FTIR and Two-Dimensional Correlation Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2113-2122. |
[15] |
DAI Li-li, SHI Guang-hai*, YUAN Ye, WANG Mei-li, WANG Yan. Infrared Spectroscopic Characteristics of Borneo and Madagascar Copal Resins and Rapid Identification between Them and Ambers with Similar Appearances[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(07): 2123-2131. |
|
|
|
|