| 光谱学与光谱分析 |
|
|
|
|
|
| Monitoring the Redox States of Thioredoxin in Protein-Protein Interaction Using Intrinsic Fluorescence Probe |
| WANG Pan1, GUO Ai-yu1, CHANG Guan-xiao2, RAN Xia1, ZHANG Yu2*, GUO Li-jun1* |
1. School of Physics and Electronics, Henan University, Kaifeng 475004, China
2. State Key Laboratory of Plant Stress, Henan University, Kaifeng 475004, China |
|
|
|
|
Abstract The cellular redox states directly affect cell proliferation, differentiation and apoptosis, and the redox states changes is particularly important to the regulation of cell survival or death. Thioredoxin is a kind of oxidation regulatory protein which is widely exists in organisms, and the change of redox states is also an important process in redox regulation. In this work, we have used the site-directed mutagenesis of protein, SDS-polyacrylamide gel electrophoresis fluorescence spectroscopy and circular dichroism etc., to investigate redox states changes between TRX (E. coli) and glutathione peroxidase(GPX3) during their interaction. By observing the fluorescence spectra of TRX and its mutants, we have studied the protein interactions as well as the redox states switching between oxidation state TRX and the reduced state GPX3. The results demonstrate the presence of interactions and electron exchanges occurring between reduced GPX3 and oxidized TRX, which is of significance for revealing the physical and chemical mechanism of TRX in intracellular signal transduction.
|
|
Received: 2014-06-25
Accepted: 2014-11-16
|
|
|
|
Corresponding Authors:
ZHANG Yu, GUO Li-jun
E-mail: juneguo@henu.edu.cn;qikeli@hotmail.com
|
|
[1] Holmgren A. Structure, 1995, 3(3): 239.
[2] Wollman E E, d’Auriol L, Rimsky L, et al. Journal of Biological Chemistry, 1988, 263(30): 15506.
[3] Arnér E S J, Holmgren A. European Journal of Biochemistry, 2000, 267(20): 6102.
[4] Koh C S, Didierjean C, Navrot N, et al. Journal of Molecular Biology, 2007, 370(3): 512.
[5] Holmgren A. Structure, 1995, 3(3): 239.
[6] Landino L M, Moynihan K L, Todd J V, et al. Biochem Bioph RES CO, 2004, 314(2): 555.
[7] Messens J, Collet J F. The International Journal of Biochemistry & Cell Biology, 2006, 38(7): 1050.
[8] Benhar M, Forrester M T, Hess D T, et al. Science, 2008, 320(5879): 1050.
[9] Barabote R D, Saier M H. Microbiology and Molecular Biology Reviews, 2005, 69(4): 608.
[10] Callister M E, Burke-Gaffney A, Evans T W, et al. Thorax, 2006, 61(6): 521.
[11] Li H, Hanson C, Fuchs J A, et al. Biochemistry, 1993, 32(22): 5800.
[12] Dorcak V, Palecek E. Analytical Chemistry, 2009, 81(4): 1543.
[13] Kumagai Y, Shinkai Y, Miura T, et al. Annual. Review of Pharmacology and Toxicology, 2012, 52: 221.
[14] Watanabe R, Nakamura H, Masutani H, et al. Pharmacol Ther., 2010, 127(3): 261.
[15] Maeda K, Hgglund P, Bjrnberg O, et al. FEBS Letters, 2010, 584(15): 3376.
[16] Kho C W, Lee P Y, Bae K H, et al. Biochem. Bioph. RES CO, 2006, 348(1): 25.
[17] Ma L H, Takanishi C L, Wood M J. Journal of Biological Chemistry, 2007, 282(43): 31429.
[18] Hixon J, Reshetnyak Y K. Algorithms, 2009, 2(3): 1155.
[19] Kyrychenko A, Posokhov Y O, Rodnin M V, et al. Biochemistry, 2009, 48(32): 7584.
[20] Vivian J T, Callis P R. Biophysical Journal, 2001, 80(5): 2093.
[21] Moon C P, Fleming K G. SProceedings of the National Academy of Sciences, 2011, 108(25): 10174.
[22] Hixon J, Reshetnyak Y K. Algorithms, 2009, 2(3): 1155.
[23] Gallego O, Betts M J, Gvozdenovic-Jeremic J, et al. Molecular Systems Biology, 2010, 6(1).
[24] Ladokhin A S, Jayasinghe S, White S H. Analytical Bbiochemistry, 2000, 285(2): 235. |
| [1] |
LEI Hong-jun1, YANG Guang1, PAN Hong-wei1*, WANG Yi-fei1, YI Jun2, WANG Ke-ke2, WANG Guo-hao2, TONG Wen-bin1, SHI Li-li1. Influence of Hydrochemical Ions on Three-Dimensional Fluorescence
Spectrum of Dissolved Organic Matter in the Water Environment
and the Proposed Classification Pretreatment Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 134-140. |
| [2] |
GU Yi-lu1, 2，PEI Jing-cheng1, 2*，ZHANG Yu-hui1, 2，YIN Xi-yan1, 2，YU Min-da1, 2, LAI Xiao-jing1, 2. Gemological and Spectral Characterization of Yellowish Green Apatite From Mexico[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 181-187. |
| [3] |
HAN Xue1, 2, LIU Hai1, 2, LIU Jia-wei3, WU Ming-kai1, 2*. Rapid Identification of Inorganic Elements in Understory Soils in
Different Regions of Guizhou Province by X-Ray
Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 225-229. |
| [4] |
WANG Hong-jian1, YU Hai-ye1, GAO Shan-yun1, LI Jin-quan1, LIU Guo-hong1, YU Yue1, LI Xiao-kai1, ZHANG Lei1, ZHANG Xin1, LU Ri-feng2, SUI Yuan-yuan1*. A Model for Predicting Early Spot Disease of Maize Based on Fluorescence Spectral Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3710-3718. |
| [5] |
CHENG Hui-zhu1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, MA Qian1, 2, ZHAO Yan-chun1, 2. Genetic Algorithm Optimized BP Neural Network for Quantitative
Analysis of Soil Heavy Metals in XRF[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3742-3746. |
| [6] |
SONG Yi-ming1, 2, SHEN Jian1, 2, LIU Chuan-yang1, 2, XIONG Qiu-ran1, 2, CHENG Cheng1, 2, CHAI Yi-di2, WANG Shi-feng2，WU Jing1, 2*. Fluorescence Quantum Yield and Fluorescence Lifetime of Indole, 3-Methylindole and L-Tryptophan[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3758-3762. |
| [7] |
YANG Ke-li1, 2, PENG Jiao-yu1, 2, DONG Ya-ping1, 2*, LIU Xin1, 2, LI Wu1, 3, LIU Hai-ning1, 3. Spectroscopic Characterization of Dissolved Organic Matter Isolated From Solar Pond[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3775-3780. |
| [8] |
LI Xiao-li1, WANG Yi-min2*, DENG Sai-wen2, WANG Yi-ya2, LI Song2, BAI Jin-feng1. Application of X-Ray Fluorescence Spectrometry in Geological and
Mineral Analysis for 60 Years[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 2989-2998. |
| [9] |
XUE Fang-jia, YU Jie*, YIN Hang, XIA Qi-yu, SHI Jie-gen, HOU Di-bo, HUANG Ping-jie, ZHANG Guang-xin. A Time Series Double Threshold Method for Pollution Events Detection in Drinking Water Using Three-Dimensional Fluorescence Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3081-3088. |
| [10] |
MA Qian1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, CHENG Hui-zhu1, 2, ZHAO Yan-chun1, 2. Research on Classification of Heavy Metal Pb in Honeysuckle Based on XRF and Transfer Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2729-2733. |
| [11] |
JIA Yu-ge1, YANG Ming-xing1, 2*, YOU Bo-ya1, YU Ke-ye1. Gemological and Spectroscopic Identification Characteristics of Frozen Jelly-Filled Turquoise and Its Raw Material[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2974-2982. |
| [12] |
YANG Xin1, 2, XIA Min1, 2, YE Yin1, 2*, WANG Jing1, 2. Spatiotemporal Distribution Characteristics of Dissolved Organic Matter Spectrum in the Agricultural Watershed of Dianbu River[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2983-2988. |
| [13] |
CHEN Wen-jing, XU Nuo, JIAO Zhao-hang, YOU Jia-hua, WANG He, QI Dong-li, FENG Yu*. Study on the Diagnosis of Breast Cancer by Fluorescence Spectrometry Based on Machine Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2407-2412. |
| [14] |
ZHU Yan-ping1, CUI Chuan-jin1*, CHENG Peng-fei1, 2, PAN Jin-yan1, SU Hao1, 2, ZHANG Yi1. Measurement of Oil Pollutants by Three-Dimensional Fluorescence
Spectroscopy Combined With BP Neural Network and SWATLD[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2467-2475. |
| [15] |
LIU Xian-yu1, YANG Jiu-chang1, 2, TU Cai1, XU Ya-fen1, XU Chang3, CHEN Quan-li2*. Study on Spectral Characteristics of Scheelite From Xuebaoding, Pingwu County, Sichuan Province, China[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2550-2556. |
|
|
|
|
