| 光谱学与光谱分析 |
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| Studies on the Interaction between Malanin and Metal Ions by Fluorescence Spectra Methods |
| YUAN Yan1, ZHANG Wei1, DAI Jian-hui1, LIU Xiao-fang1, WANG Hong-bin1, Colin J Barrow2, YANG Wen-rong2 |
1. Key Laboratory of Chemistry in Ethnic Medicinal Resources，State Ethnic Affairs Commission and Ministry of Education，Yunnan Minzu University, Kunming 650500, China
2. Center for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Geelong, VIC 3216, Australia |
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Abstract A novel protein with anti-tumor activities named malanin was isolated and purified from an endemic plant in Yunnan and Guangxi provinces. Effects of copper ion, silver ion and calcium ion on malanin and apo-malanin fluorescence spectra were studied. The results showed that copper ion leads to obvious statistic quenching of malanin and apo-malanin fluorescence. The dissociation constant of them from malanin and apo-malanin were about 2.37×10-4 and 2.66×10-4 mol·L-1, respectively. The silver ion did not have quenching action on malanin fluorescence, but it had statistic quenching effect on apo-malanin fluorescence, and its dissociation constant was 2.37×10-4 mol·L-1. Calcium ion did not have quenching action on malanin and apo-malanin fluorescence. It plays an important role in keeping malanin natural conformation.
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Received: 2013-09-11
Accepted: 2013-12-15
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Corresponding Authors:
YUAN Yan
E-mail: 54988363@qq.com
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