| 光谱学与光谱分析 |
|
|
|
|
|
| Analysis of the EEM Fluorescence Spectra of Effluent from Anaerobic Methane Reactor during the Inhibition Process |
| LI Wei-hua1, 2, SHENG Guo-ping1*, LU Rui1, ZHAO Quan-bao1, YU Han-qing1 |
1. Department of Chemistry, University of Science and Technology of China, Hefei 230026, China
2. School of Environment and Energy Engineering, Anhui Institute of Architecture Industry，Hefei 230022, China |
|
|
|
|
Abstract The effluents from the anaerobic methane reactor were characterized by the EEM fluorescence spectra. The EEM fluorescence spectra and relative concentration of the main components were acquired by PARAFAC models. Results show that the main components of the effluents from the uninhibited reactor were protein, coenzyme NADH and F420. When the methane-producing reaction was inhibited, the production rate of methane decreased rapidly. Correspondingly, the fluorescence of F420 disappeared and the main components of the reactor were protein and NADH. The fluorescence intensity scores of protein from the diluted sample were remarkably correlated to the protein determination by chemical analysis and the correlation efficient was 0.93. The fluorescence intensity scores of F420 were correlated to the production rate of methane, which implicated that coenzyme F420 could be used to reflect the activity of methane production in a certain bioreactor. This work provides a new method to monitor the operation status of anaerobic methane-producing reactor.
|
|
Received: 2010-06-25
Accepted: 2010-09-20
|
|
|
|
Corresponding Authors:
SHENG Guo-ping
E-mail: gpsheng@ustc.edu.cn
|
|
[1] Farabegoli G, Hellinga C, Heijnen J J, et al. Water Research, 2003, 37: 2732.
[2] Arunachalam R S, Shah H K, Ju L K. Water Research, 2005, 39(7): 1205.
[3] YIN Xiao-bo, LIAN Li-wen, XU Jie-quan, et al(尹小波, 连莉文, 徐洁泉, 等). China Biogas(中国沼气), 1998, 16(3): 8.
[4] TANG Yi, HU Ji-cui(唐 一, 胡纪萃). China Biogas(中国沼气), 1990, 8(1): 11.
[5] Pons M N, Bonté S L, Potier O. Journal of Biotechnology, 2004, 113(1-3): 211.
[6] Baker A. Environmental Science & Technology, 2002, 36(7): 1377.
[7] Sheng G P, Yu H Q. Water Research, 2006, 40(6): 1233.
[8] Wang Z W, Wu Z C, Tang S J. Water Research, 2009, 43(6): 1533.
[9] CHEN Mao-fu, WU Jing, Lü Yan-li, et al(陈茂福, 吴 静, 律严励, 等). Acta Optica Sinica(光学学报), 2008, 28(3): 578.
[10] Bro R. Chemometrics and Intelligent Laboratory Systems, 1997, 38(2): 149.
[11] LI Hong-bin, LIU Wen-qing, ZHANG Yu-jun, et al(李宏斌，刘文清，张玉钧，等). Journal of Atmospheric and Environmental Optics(大气与环境光学学报), 2006, 11(3): 216.
[12] WANG Zhi-gang, LIU Wen-qing, LI Hong-bin, et al(王志刚，刘文清，李宏斌，等). Journal of Environmental Sciences(环境科学学报), 2006, 26(2): 275.
[13] Li W H, Sheng G P, Liu X W, et al. Water Research, 2008, 42(12): 3173.
[14] Fang D M, Wu H L, Ding Y J, et al. Talanta, 2006, 70: 58. |
| [1] |
LEI Hong-jun1, YANG Guang1, PAN Hong-wei1*, WANG Yi-fei1, YI Jun2, WANG Ke-ke2, WANG Guo-hao2, TONG Wen-bin1, SHI Li-li1. Influence of Hydrochemical Ions on Three-Dimensional Fluorescence
Spectrum of Dissolved Organic Matter in the Water Environment
and the Proposed Classification Pretreatment Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 134-140. |
| [2] |
XIA Ming-ming1, 2, LIU Jia3, WU Meng1, 2, FAN Jian-bo1, 2, LIU Xiao-li1, 2, CHEN Ling1, 2, MA Xin-ling1, 2, LI Zhong-pei1, 2, LIU Ming1, 2*. Three Dimensional Fluorescence Characteristics of Soluble Organic Matter From Different Straw Decomposition Products Treated With Calcium Containing Additives[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 118-124. |
| [3] |
GU Yi-lu1, 2，PEI Jing-cheng1, 2*，ZHANG Yu-hui1, 2，YIN Xi-yan1, 2，YU Min-da1, 2, LAI Xiao-jing1, 2. Gemological and Spectral Characterization of Yellowish Green Apatite From Mexico[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 181-187. |
| [4] |
SONG Yi-ming1, 2, SHEN Jian1, 2, LIU Chuan-yang1, 2, XIONG Qiu-ran1, 2, CHENG Cheng1, 2, CHAI Yi-di2, WANG Shi-feng2，WU Jing1, 2*. Fluorescence Quantum Yield and Fluorescence Lifetime of Indole, 3-Methylindole and L-Tryptophan[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3758-3762. |
| [5] |
YANG Ke-li1, 2, PENG Jiao-yu1, 2, DONG Ya-ping1, 2*, LIU Xin1, 2, LI Wu1, 3, LIU Hai-ning1, 3. Spectroscopic Characterization of Dissolved Organic Matter Isolated From Solar Pond[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3775-3780. |
| [6] |
XUE Fang-jia, YU Jie*, YIN Hang, XIA Qi-yu, SHI Jie-gen, HOU Di-bo, HUANG Ping-jie, ZHANG Guang-xin. A Time Series Double Threshold Method for Pollution Events Detection in Drinking Water Using Three-Dimensional Fluorescence Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3081-3088. |
| [7] |
JIA Yu-ge1, YANG Ming-xing1, 2*, YOU Bo-ya1, YU Ke-ye1. Gemological and Spectroscopic Identification Characteristics of Frozen Jelly-Filled Turquoise and Its Raw Material[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2974-2982. |
| [8] |
YANG Xin1, 2, XIA Min1, 2, YE Yin1, 2*, WANG Jing1, 2. Spatiotemporal Distribution Characteristics of Dissolved Organic Matter Spectrum in the Agricultural Watershed of Dianbu River[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2983-2988. |
| [9] |
ZHU Yan-ping1, CUI Chuan-jin1*, CHENG Peng-fei1, 2, PAN Jin-yan1, SU Hao1, 2, ZHANG Yi1. Measurement of Oil Pollutants by Three-Dimensional Fluorescence
Spectroscopy Combined With BP Neural Network and SWATLD[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2467-2475. |
| [10] |
QIU Cun-pu1, 2, TANG Xiao-xue2, WEN Xi-xian4, MA Xin-ling2, 3, XIA Ming-ming2, 3, LI Zhong-pei2, 3, WU Meng2, 3, LI Gui-long2, 3, LIU Kai2, 3, LIU Kai-li4, LIU Ming2, 3*. Effects of Calcium Salts on the Decomposition Process of Straw and the Characteristics of Three-Dimensional Excitation-Emission Matrices of the Dissolved Organic Matter in Decomposition Products[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2301-2307. |
| [11] |
SHI Chuan-qi1, LI Yan2, HU Yu3, YU Shao-peng1*, JIN Liang2, CHEN Mei-ru1. Fluorescence Spectral Characteristics of Soil Dissolved Organic Matter in the River Wetland of Northern Cold Region, China[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1517-1523. |
| [12] |
LI Yuan-jing1, 2, CHEN Cai-yun-fei1, 2, LI Li-ping1, 2*. Spectroscopy Study of γ-Ray Irradiated Gray Akoya Pearls[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(04): 1056-1062. |
| [13] |
LIU Xia-yan1, CAO Hao-xuan1, MIAO Chuang-he1, LI Li-jun2, ZHOU Hu1, LÜ Yi-zhong1*. Three-Dimensional Fluorescence Spectra of Dissolved Organic Matter in Fluvo-Aquic Soil Profile Under Long-Term Composting Treatment[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 674-684. |
| [14] |
LÜ Yang1, PEI Jing-cheng1*, ZHANG Yu-yang2. Chemical Composition and Spectra Characteristics of Hydrothermal Synthetic Sapphire[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(11): 3546-3551. |
| [15] |
ZHANG Yong-bin1, ZHU Dan-dan1, CHEN Ying1*, LIU Zhe1, DUAN Wei-liang1, LI Shao-hua2. Wavelength Selection Method of Algal Fluorescence Spectrum Based on Convex Point Extraction From Feature Region[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(10): 3031-3038. |
|
|
|
|
