| 光谱学与光谱分析 |
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| Identification of Soil-Borne Fungi Using Fourier Transform Infrared Spectroscopy |
| CHAI A-li，LI Bao-ju*，SHI Yan-xia，XIE Xue-wen |
| Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China |
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Abstract Fourier transform infrared (FTIR) attenuated total reflectance (ATR) spectroscopy was used in combination with multivariate statistic analysis for identification of soil-borne fungi that causes severe economic damage to agriculture: Fusarium moniliforme, Fusarium semitectum, Fusarium oxysporum, Fusarium solani, Rhizoctonia solani, Sclerotinia sclerotiorum, Pythium aphanidermatum and Phytophthora capsici. The original FTIR spectra were normalized, and the second derivatives were calculated, from which the peak wave numbers showing greatest variability were selected：2 924, 2 854, 1 745, 1 641, 1 547, 1 466, 1 406, 1 376, 1 306, 1 240, 1 201, 1 152, 1 109 and 1 028 cm-1. To discriminate different fungal strains, canonical discriminant analysis and cluster analysis were performed at these characteristic wave numbers. Results showed that the classification accuracies achieved 100% for different species of fungi, and classification accuracies for different fusarium strains achieved 95.56%, demonstrating the high potential of this technique for fungi identification.
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Received: 2010-10-09
Accepted: 2011-02-17
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Corresponding Authors:
LI Bao-ju
E-mail: Libj@mail.caas.net.cn
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[1] Sivakesava S，Irudayaraj J，DebRoy C. Transactions of the ASAE，2004，7(3)：951.
[2] Greenstreet J E，Norris K P. Spectrochimica Acta，1957，9(3): 177.
[3] Naumann D，Fijala V，Labischinski H，et al. Journal of Molecular Structure，1988，174：165.
[4] Li H，Tripp C P. Applied Spectroscopy，2008，62(9)：963.
[5] Samuels A C，Snyder A P，Emge D K，et al. Applied Spectroscopy，2009，63(1)：14.
[6] Fischer G，Braum S，Thissen R，et al. Journal of Microbiological Methods，2006，64(1)：63.
[7] Naumann A. Analyst，2009，134(6)：1215.
[8] Rellini P，Roscini L，Fatichenti F，et al. FEMS Yeast Research，2009，9(3)：460.
[9] Corte L，Rellini P，Roscini L，et al. Analytica Chemica Acta，2010，659：258.
[10] Haag H，Gremlich H U，Bergmann R，et al. Journal of Microbiological Methods，1996，27(2-3)：157.
[11] Naumann D，Helm D，Labischinski H.Nature，1991，351(6321)：81.
[12] Garip S，Gozen A，Severcan F. Food Chemistry，2009，113(4)：1301. |
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