| 光谱学与光谱分析 |
|
|
|
|
|
| Studies on the Interaction of Gatifloxacin with Bovine Serum Albumin in the Presence of Carbon Nanotubes by Fluorescence Spectroscopy |
| ZHA Jun1, HE Hua1, 4*, LIU Tie-bing2, LI Shan-shan1, JIAO Qing-cai3 |
1. Division of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China
2. The Sino-Germany Institute of Agriculture Products Processing Industry, Zhejiang University of Science and Technology, Hangzhou 310023, China
3. State Key Laboratory of Pharmaceutical Biotechnology，Nanjing University，Nanjing 210093，China
4. Key Laboratory of Drug Quality Control and Pharmacovigilance of Ministry of Education(China Pharmaceutical University)，Nanjing 210009，China |
|
|
|
|
Abstract Fluorescence spectroscopy was used to investigate the influences of carbon nanotubes (CNTs) on the fluorescence of bovine serum albumin (BSA)，the influences of CNTs on that of gatifloxacin (GFLX) , and the influences of GFLX on that of BSA with or without coexisting CNTs under imitated physiological condition. The experimental results demonstrate that both gatifloxacin and carbon nanotubes could quench the intrinsic fluorescence of BSA, and the quenching mechanism is mainly static quenching. The fluorescence quenching action of GFLX on BSA was weakened in the presence of CNTs. The fluorescence quenching data were analyzed according to Stern-Volmer equation and double-reciprocal Lineweaver-Burk equation. It was shown that the quenching constant (KSV) and the binding constant (K) are decreased with the concentration of carbon nanotubes increasing. The effects of coexisting CNTs on GFLX-BSA interactions were discussed to offer a reference for the studies on the action mechanism of CNTs or GFLX with albumins in vivo.
|
|
Received: 2010-05-10
Accepted: 2010-08-20
|
|
|
|
Corresponding Authors:
HE Hua
E-mail: dochehua@163.com
|
|
[1] Iijima S. Nature, 1991, 354(6348): 56.
[2] KANG Yu，LIU Yingchun, WANG Qi，et al. Biomaterials, 2009, 30(14): 2807.
[3] LI Shan-shan，HE Hua，JIAO Qing-cai, et al(李杉杉, 何 华, 焦庆才, 等). Progress in Chemistry(化学进展)，2008，20(11)： 1798.
[4] Prato M, Kostarelos K, Bianco A. Acc. Chem. Res., 2008, 41(1): 60.
[5] Zhuang Liu, Sun Xiao-ming, Nozomi Nakayama-Ratchford. Journal of American Chemical Society, 2007, 129(1): 49.
[6] Becker M L, Fagan J A, Gallant N D, et al. Advanced Materials, 2007, 19(7): 939.
[7] Yang Shengtao, Wang Xiang, Ji Guang, et al. Toxicology Letters, 2008, 181(3)： 182.
[8] Yan Zhengyu, Shao Xiufen, Yan Lin, et al. Journal of Chinese Pharmaceutical Sciences, 2005, 14(1): 33.
[9] Callis P R. Methods Enzymol., 1997，278: 113.
[10] LI De-jun, YUAN Li, YANG Ying, et al(李德军, 袁 丽, 杨 莹, 等). Science in China(中国科学), 2009, 39(6): 596.
[11] Ashok S Kumar, Sea-Fue Wang. Materials Letters, 2009, 63: 1830.
[12] Laura E Valenti a, Pablo A Fiorito b, Carlos D García, et al. Journal of Colloid and Interface Science, 2007, 307: 349.
|
| [1] |
YI Min-na1, 2, 3, CAO Hui-min1, 2, 3*, LI Shuang-na-si1, 2, 3, ZHANG Zhu-shan-ying1, 2, 3, ZHU Chun-nan1, 2, 3. A Novel Dual Emission Carbon Point Ratio Fluorescent Probe for Rapid Detection of Lead Ions[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3788-3793. |
| [2] |
HE Yan-ping, WANG Xin, LI Hao-yang, LI Dong, CHEN Jin-quan, XU Jian-hua*. Room Temperature Synthesis of Polychromatic Tunable Luminescent Carbon Dots and Its Application in Sensitive Detection of Hemoglobin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(11): 3365-3371. |
| [3] |
LI Jin-zhi1, LIU Chang-jin1, 4*, SHE Zhi-yu2, ZHOU Biao2, XIE Zhi-yong2, ZHANG Jun-bing3, JIANG Shen-hua2, 4*. Antiglycation Activity on LDL of Clove Essential Oil and the Interaction of Its Most Abundant Component—Eugenol With Bovine Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(01): 324-332. |
| [4] |
SHAO Ke-man, FU Gui-yu, CHEN Su-yan, HONG Cheng-yi, LIN Zheng-zhong*, HUANG Zhi-yong*. Preparation of Molecularly Imprinted Fluorescent Probe for Rare Earth Complex and Determination of Malachite Green Residue[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(03): 808-813. |
| [5] |
WANG Jun1, WANG Zhou-li2, CHENG Jing-jing1. Interaction Between Tartrazine and Bovine Serum Albumin Using Multispectral Method and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(03): 904-909. |
| [6] |
WANG Xiao-xia1,3*, WU Hao1, NIE Zhi-hua2, MA Li-tong1,3*, CUI Jin-long1, SAI Hua-zheng1, CHENG Jian-guo1. Study on the Interaction Between Fulvic Acid and Bovine Serum Albumin by Multispectral and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(09): 2904-2910. |
| [7] |
ZHANG Chuan-ying1, PENG Xin1*, RAO Heng-jun2, QI Wei2, SU Rong-xin2, HE Zhi-min2. Spectroscopic Studies on the Interaction Between Salvianolic Acid B and Bovine Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(06): 1701-1707. |
| [8] |
CHEN Jian1, HUANG Jun-shi1, 2, LIU Mu-hua1, 2, YUAN Hai-chao1, 2, HUANG Shuang-gen1, 2, ZHAO Jin-hui1, 2*, XU Ning1, WANG Ting1, HU Wei1. Study on Rapid Detection Method of Danofloxacin Mesylate and Ofloxacin Residues in a Chicken Based on Synchronous Fluorescence Spectrum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(05): 1367-1372. |
| [9] |
TUO Xun1, SONG Ji-min1, FU Hao2, LÜ Xiao-lan1*. Study on Interaction Between Hexabromocyclododecane and Bovine Serum Albumin by Spectroscopy and Computer Simulation Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(05): 1487-1492. |
| [10] |
LI Xue1, WANG Li1, LIU Guang-xian1*, TU Zong-cai2. Effect of Urea on Glycosylation of BSA Based on Spectral Techniques[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(02): 478-483. |
| [11] |
LI Xing-xing, ZHANG Xiang, HUANG Xue-song*. Interaction Between Three Sulfur-Containing Amino Acids in Garlic and Bovine Serum Albumin Determined by Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(11): 3483-3488. |
| [12] |
JIA Hui-jie, ZHU Ning, GAO Yuan-yuan, WANG Ya-qi, SUO Quan-ling*. Effect of Substituent Structure of Benzothiazole Probe on Recognition to Metal Ion[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(11): 3594-3598. |
| [13] |
ZHANG Wei-wei, SUN Yan-hui*, GU Hai-yang, LÜ Ri-qin, WANG Wen-zheng. Application of Synchronous Fluorescence Spectroscopy and Chemometric Models for Rapid Evaluating Peanut Oil Oxidative Stability[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(10): 3113-3117. |
| [14] |
HUANG Fang1, LIU Ming-xue1, 2*, XIONG Jie1, CHEN Lü-qi1, GAO Zhu-xin1, CHEN Hui-ming1, WANG Dan-ni1. Effect of Far-Infrared Ceramic Powder on the Interaction Between Essential Oil and BSA[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(08): 2358-2365. |
| [15] |
LI Chong-wei1, 2, YU Hui-bin2*, YANG Fang2, GUO Xu-jing1, GAO Hong-jie2, BAI Yang2. Applying SFS, FTIR and 2D-COS to Analyze Structural Compositions of DOM in Aquatic System, and Reveal Their Special Variation[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(07): 2005-2010. |
|
|
|
|
