| 光谱学与光谱分析 |
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| Determination of Deoxyribonucleic Acid with Nitro-Tetrazolium Chloride Blue by Resonance Light-Scattering Technique |
| LONG Yun-fei1，2，CHEN Xiao-ming1*，CAI Tie-jun2 |
1. Institute of Chemistry, Xiangtan University，Xiangtan 411105，China
2. Department of Chemistry, Xiangtan Normal University, Xiangtan 411201, China |
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Abstract Characteristics of the interaction of nitro-tetrazolium chloride blue and Deoxyribonucleic Acid(DNA) were studied in the of pH 1.8 buffer solution by Resonance Light-Scattering (RLS) technique. The effects of interference substances and the optimization of the procedure were investigated，and a new method for the determination of the DNA was established based on RLS. The linear range of the DNA is 0-7 mg·L-1 with a correlation coefficient of 0.995 8. The detection limit is 30.04 μg·L-1. The method has been applied to the determination of DNA in mixed samples with satisfactory result.
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Received: 2002-10-16
Accepted: 2003-01-26
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Corresponding Authors:
CHEN Xiao-ming
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| Cite this article: |
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LONG Yun-fei,CHEN Xiao-ming,CAI Tie-jun. Determination of Deoxyribonucleic Acid with Nitro-Tetrazolium Chloride Blue by Resonance Light-Scattering Technique [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(05): 610-612.
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| URL: |
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http://www.gpxygpfx.com/EN/Y2004/V24/I05/610 |
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