| 光谱学与光谱分析 |
|
|
|
|
|
| Resonance Scattering Spectral Detection of Trace K+ by Aptamer-Modified Nanogold Probe |
| LIU Qing-ye, FAN Yan-yan, LI Ting-sheng, LIANG Ai-hui, JIANG Zhi-liang* |
| Key Laboratory of Guangxi Environmental Engineering and Protection Evaluation, School of Environment and Resource Sciences, Guangxi Normal University，Guilin 541004, China |
|
|
|
|
Abstract In pH 7.0 Na2HPO4-NaH2PO4 buffer solution, nanogold particles interacted with the aptamer to form a stable aptamer-nanogold complex that was not aggregation by NaCl. At 80 ℃, K+ and aptamer folded to form a stable G-quadruplex that released nanogold particles, the uncombined nanogold particles aggregated to large nanogold clusters that caused the increase in resonance scattering (RS) intensity at 563 nm in high concentration of NaCl, and the laser scattering showed that the average diameter was 120 nm. In the present paper, the resonance scattering spectral characteristics of K+-ssDNA1-Au, K+-ssDNA2-Au and K+-aptamer-Au systems were investigated, and the structural changes of aptamer were studied by circular dichroism spectral technology. Effects of pH value，NaCl concentration，nanogold concentration，aptamer concentration，and the reactation temperature and time on the resonance scattering intensity were considered in detail. The influence of coexistent substances on the determination of K+ was investigated, result showed that the common heavy metal ions such as Cu2+, Mg2+, Pb2+, Ca2+, Al3+, Zn2+ and Fe3+do not interfere with the determination, and the method has good selectivity. Under the conditions selected, a 0.67-3 350 μmol·L-1 K+ can be detected by the aptamer-nanogold RS assay, with a detection limit of 0.3 μmol·L-1 K+, regression equation ΔI=0.167c-0.7, and a coefficient of 0.993 2. The method was used for analysis of K+ in serum sample with the results consistent with the ion-selective electrode method.
|
|
Received: 2010-01-28
Accepted: 2010-04-29
|
|
|
|
Corresponding Authors:
JIANG Zhi-liang
E-mail: zljiang@mailbox.gxnu.edu.cn
|
|
[1] Saulis G, Saulius S, Rita P. Anal. Biochem., 2007, 360: 273.
[2] Gareth J P, Philip L D. Sens. Actuat. B, 2006, 114: 466.
[3] Mariusz P, Mark E M. Anal. Chem., 2009, 81: 5961.
[4] Kong D M, Guo J H, Yang W, et al. Biosens. Bioelectron., 2009, 25: 88.
[5] Wang L H, Liu X F, Hu X F, et al. Chem. Commun., 2006, 3780.
[6] Abd-Elgawad R, Ciara K O. Chem. Commun., 2006, 3432.
[7] LI Yi-lin, GUO Lei, ZHANG Zhao-yang, et al(李一林, 郭 磊, 张朝阳, 等). Science in China Series B: Chemistry(中国科学B辑, 化学), 2008, 38: 1.
[8] Huang C C, Chang H T. Chem. Commun., 2008, 1461.
[9] German I, Buchanan D D, Kennedy R T. Anal. Chem., 1998, 70: 4540.
[10] Wang X L, Li F, Su Y H, et al. Anal. Chem., 2004, 76: 5605.
[11] Feng K J, Kang Y, Zhao J J, et al. Anal. Biochem., 2008, 378: 38.
[12] Han Z Q, Qi L, Shen G Y, et al. Anal. Chem., 2007, 79: 5862.
[13] WEI Xiao-ling, ZHANG Yu-lan, JIANG Zhi-liang, et a1(韦小玲, 张玉兰, 蒋治良). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2009, 29(6): 1640.
[14] Jiang Z L, Fan Y Y, ChenM L, et al. Anal. Chem., 2009, 81: 5439.
[15] Jiang Z L, Liao X J, Deng A P, et al. Anal. Chem., 2008, 80: 8681.
[16] DONG Zhen-nan, TIAN Ya-ping, LI Guo-jun(董振南, 田亚平, 李国君). Analtical Instrumentation(分析仪器), 2002, (1): 36.
|
| [1] |
CONG Jian-han1, LUO Yun-jing1*, QI Xiao-hua2, ZOU Ming-qiang2, KONG Chen-chen1. Sensitive Detection of Uric Acid Based on BSA Gold Nanoclusters by Fluorescence Energy Resonance Transfer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(02): 483-489. |
| [2] |
JIANG Li-ying, XU Xiao-ping, QIN Zi-rui, ZHANG Pei, MENG Xiao-long, REN Lin-jiao*, WANG Wei*. A Fluorescence Aptasensor for Detecting Silver Ion Concentration in Aqueous Environment[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(04): 1066-1071. |
| [3] |
LIU Xiao-hong1,2, DENG Hua1, CHANG Lin2, ZHANG Wei2, JIANG Shan2*. Recent Progress of SERS for Environmental Estrogen Detection[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(10): 3038-3047. |
| [4] |
TENG Yuan-jie, WEI Qi-zhen, LIU Wen-han, LIU Jiang-mei, NIE Yong-hui, LI Pan. Cy3-Labeled Aptamer Combined with Surface-Enhanced Raman Scattering Using for Specific Detection of Trace Acetamiprid[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(08): 2462-2467. |
| [5] |
ZHAO Chen, HONG Cheng-yi, LIN Zheng-zhong, HUANG Zhi-yong*. Colorimetric Detection of Trace Malachite Green by Label-Free RNA-Aptamer and AuNPs[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(03): 831-836. |
| [6] |
HAO Xiao-jian*, TANG Hui-juan, HU Xiao-tao. Detection Sensitivity Improvement Study of LIBS by Combining Au-Nanoparticles and Magnetic Field[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(05): 1599-1603. |
| [7] |
LI Dan-dan1, 2, CHEN Xin3, WANG Hong3, FU Yang1, 2, YU Yuan1，ZHI Jin-fang1，2*. Visualization of the Interaction between NDs and Cells with 3D Raman Imaging[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(09): 2770-2777. |
| [8] |
LIANG Ai-hui, SHANG Guang-yun, ZHANG Xing-hui, WEN Gui-qing, JIANG Zhi-liang* . A Facile Nanogold Surface Plasmon Resonance Absorption Method for CO Tracing[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(08): 2576-2578. |
| [9] |
ZENG Hong-juan, ZHAO Ran-lin, WANG De-shun, LI Cai-xia, LIU Yi-yao . Optical Analysis of the Interaction of Mercaptan Derivatives of Nanogold Particles with Carcinoembryonic Antigen[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(02): 478-481. |
| [10] |
LIU Xiao-zhen1, FENG Jun-liang1, LIU Xiao-zhou2, CHEN Jie3, ZHU Wan-wan1, GUI Xiu-min1 . Effect of REAu on Silver Staining Results and Their UV-Vis Absorption Spectra[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(12): 3560-3564. |
| [11] |
XU Xue-qin1, LIU Qiong-hua1, YANG Fang2*, QIAN Jiang2, CHEN Jian2, LIN Zhen-yu1, QIU Bin1 . Preparation of Gold Nano-Particles as Surface-Enhanced Raman Scattering Sensors for Analysis of Banned Food Dye Chrysoidin in Yuba[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(11): 3092-3095. |
| [12] |
MA Xiao-yuan1, 2, 3, LIU Ying1, WANG Zhou-ping1, 3* . Preparation, Characterization and Surface-Enhanced Raman Properties of Agarose Gel/Gold Nanoparticles Hybrid [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(08): 2126-2131. |
| [13] |
LIU Jin-chuan, Lü Zhen-zhen, CHEN Ai-liang* . Gold Nanoparticle-Aptamer Based Colorimetric Biosensing Assays [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(08): 2040-2046. |
| [14] |
YAO Cui-ping1*, WANG Jing1*, YANG Yang1, DONG Yan-hua1, XUE Yang1, MEI Jian-sheng1, ZENG Wei-hui2, ZHANG Zhen-xi1*. Synthesis of Folate Receptor-Targeted Nanoprobe for Detection of Cancer Cells and Its Spectral Analysis [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(05): 1299-1303. |
| [15] |
WANG Sheng-mian1, 2, WU Meng1, LIANG Ai-hui1*, JIANG Zhi-liang1* . DNAzyme Cracking-Nanogold Resonance Rayleigh Scattering Spectral Method for the Determination of Trace Cu2+ [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(01): 147-150. |
|
|
|
|
