木素小分子愈创木基型松柏醇漆酶生物改性的光谱分析

Abstract
References
Related Citation (15)

Download: PDF (1381 KB)
Export: BibTeX | EndNote (RIS)

Abstract The enzymatic modification mechanism of lignin small molecular lignin guaiacyl coniferyl alcohol existing in softwood and hardwood treated by laccase was studied. Gas chromatography-mass spectrometry (GC-MS), gel permeation chromatography (GPC), FTIR spectrum and PCD(particle charge detector) etc were used for the measurement. GC-MS could not detect the coniferyl alcohol monomer after treated by laccase, so it was speculated that coniferyl alcohol participated in the reaction totally, and the structure of coniferyl alcohol was changed. GPC demonstrated that the molecular weight of coniferyl alcohol increased when it was treated by laccase, and coniferyl alcohol was polymerized. FTIR spectrum determined the reaction point of coniferyl alcohol when treated by laccase, and it is mainly phenolic hydroxyl，aromatic ring side chain substituent such as methoxy, double bound in side chain, β-carbonyl groups, and γ-carbonyl groups. PCD gave a result that the cationic demandv(CD) decreased by 88.38%.

Key words：Coniferyl alcohol;Gas chromatography-mass spectrometry;Gel permeation chromatography;FTIR spectra;Laccase

Received: 2009-06-28 Accepted: 2009-09-28

ZTFLH:	O657
	O636.2

Corresponding Authors: PEI Ji-cheng E-mail: jcpei@tust.edu.cn

Cite this article:

LIU Hai-tang,PEI Ji-cheng,HU Hui-ren, et al. Spectra Analysis of Lignin Small Molecular Guaiacyl Coniferyl Alcohol Biological Modification Treated by Laccase [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(06): 1469-1473.

URL:

https://www.gpxygpfx.com/EN/Y2010/V30/I06/1469

[1] LIU Na，SHI Shu-lan(刘娜，石淑兰). Journal of Cellulose Science and Technology(纤维素科学与技术), 2005, 13(3): 56.
[2] Sjostrom J. Nord. Pulp Paper Res. J, 1990, 5(1): 9.
[3] Seika Tay. Tappi. Journal, 2001, 84(8): 1.
[4] Ben Y, Dorris G, Hill G, et al. Pulp & Paper Canada, 2003, 104(1): 42.
[5] Wagberg L, Asell I. Nordic Pulp Paper Res J, 1991, 6: 127.
[6] Leonowicz A, Edgehill R U, Bollag J M. Arch. Microbiol., 1984, 137: 89.
[7] Sannia Giovanni, Giardina Paola, Palmieri Gianna, et al. WO0212136 (2002-02-14).
[8] Livernoche D, Jurasek L, Desrohers M, et al. Biotechnology and Bioengineering, 1983, 25: 2055.
[9] Archibald Frederick, Paice Michael C, Lubomir Jurasek. Enzyme Micrb Technol., 1990, 12(11): 846.
[10] Pratima Bajpal. Biotechnol. Prog., 1999, 15: 147.
[11] Johan Gullichsen, Hannu Paulapuro. Forest Products Chemistry(Book 3) In：Papermaking Science and Technology. Published in Cooperation with the Finnish Paper Engineer’s Association and Tappi, 2001.
[12] ZHOU Peng, SHEN Jin-can, XIE Ming-yong, et al(周鹏, 沈金灿, 谢明勇, 等). Journal of Xiamen University(Natural Science)(厦门大学学报·自然科学版), 2003, 42(2): 211.
[13] Carsten Mai, Wiebke Schormanna, Alois Hüttermanna. Enzyme and Microbial Technology, 2001, 4-5(28): 460.
[14] QIU Wei-hua, CHEN Hong-zhang(邱卫华，陈洪章). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2008，28(7)，1501.
[15] DENG Yao-jie, LIN Lu, ZHAN Huai-yu, et al(邓耀杰，林鹿，詹怀宇，等). China Pulp & Paper Industry(中华纸业)，1998(3)：21.
[16] Wuhan University，Xiamen University，Nankai University，et al(武汉大学，厦门大学，南开大学，等). Analytical Chemistry(Fifth Edition) Volumne 2(分析化学第5版, 下册). Beijing: Higher Education Press(北京：高等教育出版社), 2007.
[17] XUE Song(薛松). Organic Structural Analysis(有机结构分析). Hefei: University of Science and Technology of China Press(合肥：中国科学技术大学出版社)，2005.
[18] KONG Chui-hua, XU Xiao-hua(孔垂华，徐效华). The Separation and Structure Identification of Organic(有机物的分离和结构鉴定). Beijing: Chemical Industry Press(北京：化学工业出版社)，2003.
[19] YANG Shu-hui(杨淑蕙). Plant Fiber Chemistry(The Third Edition)(植物纤维化学, 第3版). Beijing: China Light Industry Press(北京：中国轻工业出版社)，2001.
[20] Tejado A, Pena C, Labidi J, et al. Bioresource Technology, 2007, 98(8): 1655.
[21] SHI Shu-lan, HE Fu-wang(石淑兰，何福望). The Pulping and Papermaking Analysis and Detection(制浆造纸分析与检测). Beijing: China Light Industry Press(北京：中国轻工业出版社)，2003. 294.