| 光谱学与光谱分析 |
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| The Interaction of Vitamin B6 with the Human Serum Albumin |
| WANG Jian-lin2, FU Lian-chun3, ZHOU Shi-wu4, CHEN Zhi-jian1, Lü Wen-bo1, YE Xue-min1, MENG Guang-zheng5, SONG Zeng-fu1* |
1. School of physics, Peking University, Beijing 100871, China
2. College of Material Science and Chemical Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China
3. CTA-Tex Chemicals, Beijing 100025, China
4. College of Physical Science and Technology, Yangzhou University, Yangzhou 225002, China
5. Central Iron and Steel Research Institute, Beijing 100081, China |
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Abstract The Human Serum Albumin(HSA)can emit fluorescence(λem=350 nm)under irradiation of ultraviolet light(λex=296 nm). After the vitamin B6(B6)was added into HSA solution the fluorescence of HSA was quenched partially. This quenching was static quenching owing to the fact that τ０(fluorescence life time (life time of the molecule excitation state) of HSA without B6) equaled to τi(fluorescence life time of HSA with B6). The formation constant K between HSA and B6 was observed from the experiment, and then the binding distance R０ was determined(R０=1.872 nm). The CD spectra of samples (HSA，=1×10-5 mol·L-1，HSA+B6，[B6]=5×10-8，1.5×10-7，2.5×10-7，3.5×10-7，4.5×10-7 mol·L-1) were measured, and all CD spectra were almost the same. From [θ]values, the contents(percent content)of four structure(α-Helix，β-Pleated，β-corner，and Randon wind)of samples can be calculated, and we find that all samples contain almost the same structure contents.
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Received: 2004-12-08
Accepted: 2005-02-28
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Corresponding Authors:
SONG Zeng-fu
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| Cite this article: |
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WANG Jian-lin,FU Lian-chun,ZHOU Shi-wu, et al. The Interaction of Vitamin B6 with the Human Serum Albumin [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(06): 912-915.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2005/V25/I06/912 |
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