光谱学与光谱分析 |
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Synthesis of Substituted 3H-Indole Quaternary Ammonium Salt and Study on Its Interaction with Bovine Serum Albumin by Fluorescence |
XU Tong-kuan, SHEN Xing-hai*, GAO Hong-cheng |
College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China |
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Abstract A substituted 3H-indole quaternary ammonium molecule was designed and synthesized using hexamethylphosphoramide (HMPA) as a solvent. The products were purified and characterized by IR, 1H-NMR, MS and elemental analysis. The binding reaction of this compound with bovine serum albumin (BSA) in aqueous solution was studied using fluorescence. Their binding constant is Ka=1.995×105 dm3·mol-1 and the binding site number is n=1.12. It is confirmed that the combination is a single static quenching process.
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Received: 2003-10-28
Accepted: 2004-01-08
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Corresponding Authors:
SHEN Xing-hai
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Cite this article: |
XU Tong-kuan,SHEN Xing-hai,GAO Hong-cheng. Synthesis of Substituted 3H-Indole Quaternary Ammonium Salt and Study on Its Interaction with Bovine Serum Albumin by Fluorescence [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(06): 857-860.
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URL: |
https://www.gpxygpfx.com/EN/Y2005/V25/I06/857 |
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