光谱学与光谱分析 |
|
|
|
|
|
Study on the Interaction of Water-Soluble p-(N, N-Dimethylaminomethyl)calix[8]arene with DNA by Fluorescent Spectrometry |
LI Lai-sheng, HUANG Zhi-bing, WANG Yu-xiao,LIU Xu,LI Yan-ping,GE Xiao-hui |
The Center of Analysis and Testing, Nanchang University, Nanchang 330047, China |
|
|
Abstract The interaction of water-soluble p-(N, N-dimethylaminomethyl)calix[8]arene (CX8) with DNA was studied using adriamycin(ADM) as a probe by fluorescent spectrometry.The effect factors such as, pH of solution, salt effect and unlinking DNA on the spectra were also investigated.The interaction mechanism was proposed.The authors observed that the fluorescence of ADM was quenched by calf thymus DNA, and the fluorescence intensity of DNA-ADM enhanced with the increasing of the CX8 gradually.The result indicates that the strong electrostatic interaction between the oxyphosphate anion of DNA and the CX8 existed.According to the plot of scatchard, the results imply that the influence of CX8 on interaction of DNA-ADM belongs to mixed model, on the one hand, in the condition of neutral and acidity, the oxyphosphate anion of DNA can be neutralized by CX8 partially, which induced the contraction of DNA and the conformational change of DNA, which led to release ADM from DNA partially,on the other hand, the CX8 can also compete the electrostatic sites with ADM directly.
|
Received: 2004-03-18
Accepted: 2004-05-28
|
|
Corresponding Authors:
LI Lai-sheng
|
|
Cite this article: |
LI Lai-sheng,HUANG Zhi-bing,WANG Yu-xiao, et al. Study on the Interaction of Water-Soluble p-(N, N-Dimethylaminomethyl)calix[8]arene with DNA by Fluorescent Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(07): 1088-1091.
|
|
|
|
URL: |
https://www.gpxygpfx.com/EN/Y2005/V25/I07/1088 |
[1] Zhang L, Godinez L A, et al.Angew, Chem.Int.Ed.Engl, 1995, 34: 235. [2] Alam I, Gutsche C D.J.Org.Chem., 1990, 55: 4487. [3] Shikai S, Kawabata H, Matsuda T.Bull.Chem.Soc.Japan, 1990, 63: 1272. [4] Arnaud N F, Fuangswasdi S, Notti A, et al.Angewandte Chemie, 1998, 37: 112. [5] Yamamoto H, Shinkai S.Chem.Lett., 1994, (6): 1115.[6] HAN Bao-hang, LIU Yu, CHEN Rong-ti(韩宝航,刘 育,陈荣悌).Acta Chim.Sinica(化学学报), 2001, 59: 550. [7] LI Wen-you, XU Jin-gou(李文友,许金钩).Analytical Chemistry: Achievements and Challenges(分析化学的成就与挑战).Chongqing: Southwest Normal University Press(重庆: 西南师范大学出版社), 2000.371. [8] Gutsche C D, Nam K C.J.Am.Chem.Soc., 1988, 110: 6153. [9] LI An-zhi, DING Mei, YU Hai-ying,et al(李安之,丁 玫,于海鹰,等).Acta.Physico-Chimica Sinica(物理化学学报), 1992, 8(2): 207. [10] LI Lai-sheng, HUANG Wei-dong, YAN Yuan(李来生,黄伟东,鄢 远).Spectroscopy and Spectral Analysis(光谱学与光谱分析),2001,21(5): 685. [11] GUO Mao-lin, YANG Pin, YANG Bin-sheng, et al(郭茂林,杨 频,杨斌盛,等).Chinese Science Bulletin(科学通报), 1995, 40(13): 1187. [12] JIN Lan, YANG Pin, LI Qing-shan(靳 兰,杨 频,李青山).Chem.J.Chinese Univ.(高等学校化学学报), 1996, 17(9): 1345. [13] SHEN Jing-shan, SUN Dan-dan, FU Lian-chun, et al(沈景山,孙丹丹,付连春,等).Spectroscopy and Spectral Analysis(光谱学与光谱分析),2005,25(2): 232.
|
[1] |
ZHU Shao-hao1, SUN Xue-ping1, TAN Jing-ying1, YANG Dong-xu1, WANG Hai-xia2*, WANG Xiu-zhong1*. Study on a New Sensing Method of Colorimetric and Fluorescence Dual Modes for Pesticide Residue[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2785-2791. |
[2] |
GUO He-qing1, 2, ZHANG Sheng-zi2*, LIU Xiao-meng2, JING Xu-feng1, WANG Hong-jun2. Research Progress of the Real-Time Detection System of Bioaerosols Based on Fluorescence Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2339-2347. |
[3] |
OU Li-juan1*, LI Jing1, ZHANG Chao-qun1, LUO Jian-xin1, WEI Ji1, WANG Hai-bo2*, ZHANG Chun-yan1. Redox-Controlled Turn-on Fluorescence Sensor for H2O2 and Glucose Using DNA-Template Gold Nanoclusters[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(12): 3757-3761. |
[4] |
LIU Pan1, 2, DU Mi-fang1, LI Zhi-ya1, GAO Ling-qing1, 3, HAN Hua-yun4, ZHANG Xin-yao1, 3. Determination of Trace Tellurium Content in Steel by Hydride Generation Atomic Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(10): 3103-3108. |
[5] |
OU Li-juan, AN Xue-zhong, LUO Jian-xin, WANG Ling-yun, BO Heng, SUN Ai-ming, CHEN Lan-lan. High-Sensitive and Rapid Fluorescencet Detection of Hg2+ Based on Poly(adenine)-Templated Gold Nanoclusters[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(01): 164-167. |
[6] |
GONG Ai-qin1*, JIN Dang-qin1, ZHU Xia-shi2. A Fluorescence Spectroscopic Study of the Interaction between Trelagliptin Succinate and Bovin Serum Albumine and Its Analytical Application[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(01): 157-160. |
[7] |
MA Xue-hong1, SONG Bai-ling2, XIAO Wen-jun2, GENG Jing2, LI Xin-xia1*, CHEN Jian3. Study on Interaction between Alliin and Bovine and Hunman Serum Albumin with Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(06): 1826-1830. |
[8] |
ZHANG Li-hua1, Arkin IBURAIM2* . The Establishment of the Method of the Fiber Optic Chemical Sensor Synchronous Absorption-Fluorescence[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(03): 755-758. |
[9] |
XIONG Chao1,2, GE Liang-quan1*, LIU Duan3, ZHANG Qing-xian1, GU Yi1, LUO Yao-yao1, ZHAO Jian-kun1 . Influence of the Experiment Energy Dispersive X-Ray Fluorescence Measurement of Uranium by Different Excitation Source[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(03): 838-841. |
[10] |
LIANG Ai-hui1, LI Yuan1, HUANG Shan-shan1, LUO Yang-he1, 2, WEN Gui-qing1, JIANG Zhi-liang1* . Fluorescence Determination of Trace Se with the Hydride-KI3-Rhodamine 6G System [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2015, 35(05): 1306-1308. |
[11] |
WANG Ji-xun, GAO Xun*, LI Qi, ZHENG Yi-ni, LIN Jing-quan . Study of the Air Plasma Expansion Dynamics by Fluorescence Method [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(09): 2472-2475. |
[12] |
ZHOU Na1, 2, LUO He-dong2, LI Na1, 2, LI Yao-qun1* . Determination of Benzo(α)pyrene in Food with Microwave-Assisted Extraction[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(03): 787-790. |
[13] |
WANG Yan-yan1, SHI Zhi-qiang2, ZHANG Biao2 . Determinatoin of Trace Mercury in Sludge Samples by Hydride Generation-Atomic Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2013, 33(03): 826-828. |
[14] |
WANG Jing, XIAO Ya-ping*, LIANG Xiao-qing, SHAO Xian-hui, ZHANG Ke . Determination of Arsenic, Mercury and Selenium in Gynostemma Pentaphyllum and Rhizospheric Soil Samples Collected from Different Regions by Hydride Generation Atomic Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(03): 813-816. |
[15] |
WU Cheng-ke1, DONG Qiu-peng1,2, ZHANG Hong1, XIONG Cen1, WU Xiu-li1, LING Lian-sheng1* . Spectral Study on the Interaction of [Cu(DPPZ)(L-Ser)]+ Complex with DNA and Its Analytical Application [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(04): 1057-1060. |
|
|
|
|