有/无金属离子Cu<SUP>2+</SUP>或Zn<SUP>2+</SUP>参与时中药有效成分伞形花内酯与生物大分子BSA的相互作用

Abstract
References
Related Citation (15)

Download: PDF (579 KB)
Export: BibTeX | EndNote (RIS)

Abstract The interaction between bovine serum albumin (BSA) and umbelliferone, an active component of Chinese herbs, with or without metal ions was investigated using fluorescence spectroscopy (FS) and UV.The experimental results showed that the umbelliferone molecules inserted into the hydrophobic pockets of BSA and quenched the intrinsic fluorescence of BSA by forming umbelliferone-BSA complex.The mechanism of fluorescence quenching was confirmed combining by both static quenching and nonradiative energy transferring.It was discovered that the apparent association constant (K_A) increases in the presence of Cu²⁺ and Zn²⁺, whereas the spatial-distance (r) between umbelliferone molecule and fluorescent amino acid residues of the BSA and the binding sites (n) of umbelliferone molecules on BSA have no obviously change.Binding of umbelliferone molecules to BSA was a spontaneous supramolecular interaction in which entropy increased and Gibbs free energy decreaed.The interaction of the umbelliferone-BSA was driven mainly by electrostatic force which was enhanced by Cu²⁺ and Zn²⁺, thus the contribution of ΔH to ΔG increased in the presence of metal ions.

Key words：Active component of Chinese herbs;Umbelliferone;Bovine serum albumin;Metal ion;Fluorescence spectroscopy

Received: 2003-12-08 Accepted: 2004-03-28

ZTFLH:

O657

Corresponding Authors: FANG Yun

Cite this article:

LIU Xue-feng,XIA Yong-mei,FANG Yun, et al. Interaction between Bovine Serum Albumin and Umbelliferone with/without Metal Ions of Cu²⁺ or Zn²⁺[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(08): 1301-1305.

URL:

https://www.gpxygpfx.com/EN/Y2005/V25/I08/1301

[1] LIU Bao-sheng, ZHANG Hong-yi, WANG Fu-li(刘保生，张红医，王甫丽).Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2003，23(3)： 539.
[2] ZHANG Hong-yi, LIU Bao-sheng, WANG Fu-li(张红医，刘保生，王甫丽).Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2003，23(2)： 342.
[3] FENG Xi-lan, LI Na, ZHAO Feng-ling，et al(冯喜兰，李娜，赵凤林，等).Acta Chimica Sinica(化学学报)，2003, 61： 603.
[4] Jiang C Q, Gao M X, Meng X Z.Spectrochimica Acta Part A, 2003, 59： 1605.
[5] Il'ichev Y V; Perry J L, Simon J D.J.Phys.Chem.B, 2002, 106： 452.
[6] Il'ichev Y V; Perry J L, Simon J D.J.Phys.Chem.B, 2002, 106： 460.
[7] LIU Yuan, XIE Meng-xia, KANG Juan(刘媛，谢孟峡，康娟).Acta Chimica Sinica(化学学报), 2003, 61： 1305.
[8] ZHANG Li-wei，YANG Pin，WANG Fang(张立伟, 杨频, 王芳).Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2001，21(5)： 694.
[9] Shobini J, Mishra A K, Sandhya K，et al.Spectrochimica Acta Part A, 2001, 57: 1133.
[10] LI Jia-rong(李嘉蓉).Chinese Traditional Medicines and Herbal Drug(中草药), 1982, 13： 13.
[11] YU Tian-zhi, TAO Zu-yi(俞天智，陶祖贻).Spectroscopy and Spectral Analysis(光谱学与光谱分析), 1999, 19(3)： 453.
[12] JIANG Yun-bao, XU Jin-gou, CHEN Guo-zhen(江云宝，许金钩，陈国珍).Chinese Journal of Analytical Chemistry(分析化学), 1992, 20： 1109.
[13] Gemlamo E L, Silva C H T P, Imasato H, et al.Biochimica et Biophysica Acta, 2002, 1594： 84.
[14] YANG Pin, GAO Fei(杨频，高飞).The Principles of Bioinorganic Chemistry(生物无机化学原理).Beijing: Science Press(北京: 科学出版社), 2002.
[15] GAO Hong-wen, CHEN Yun-sheng(郜洪文, 陈运生).Chem.J.of Chinese Universities(高等学校化学学报)，2002，23(5)： 825.
[16] YI Ping-gui, LIU Jun-feng, SHANG Zhi-cai，et al(易平贵，刘俊峰，商志才，等).Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2001, 21(6)： 826.
[17] Ross D P, Sabramanian S.Biochemistry, 1981, 20： 3096.
[18] Xia J L, Paul L D, Yesook K.J.Phys.Chem., 1992, 96: 6805.