| 光谱学与光谱分析 |
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| Determination the Chemical Speciation of Cu，Zn，Fe and Mn in Radix Scutellariae by AAS |
| MIAO Shan1, SUN Ji-yuan1, XIE Yan-hua1, WANG Jian-bo1, SHI Xiao-peng2, DING Yuan-yuan1, BI Lin-lin1, GAO Shuang-bin2, WANG Si-wang1* |
1. Institute of Materia Medica，School of Pharmacy, Fourth Military Medical University, Xi’an 710032 China
2. Department of Pharmacy of Xijing Hospital，Fourth Military Medical University，Xi’an 710032 China |
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Abstract An analysis method was developed to determine the chemical speciation of Cu, Zn, Fe and Mn in radix scutellariae decoction using atomic absorption spectroscopy(AAS). The decoction can be divided into suspension and soluble species by 0.45 μm filter membrane and the soluble species can be separated into organism and inorganic species by LSA-10 macroporous resin. These elements in water-soluble test samples can be divided into alcohol-soluble and water-soluble by adopting n-octyl alcohol-water allocation system in man-made gastric acidity. Then, the concentration of these elements was determined by AAS, which provided more chemical speciation information about these elements instead of the total amount of them only in radix scutellariae. Detection limit of Cu, Zn and Mn by using the method was all 0.01 μg·mL-1 and was 0.02 μg·mL-1 for Fe. The RSD was in the range of 1.5％-3.6％(n=11) and the recovery rate of soluble species and inorganic species were in range of 96.7％-105.0％. The method has been successfully applied to determine the chemical speciation of Cu, Zn, Fe and Mn in radix scutellariae, which was very important for overall study of radix scutellariae.
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Received: 2007-11-12
Accepted: 2008-02-28
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Corresponding Authors:
WANG Si-wang
E-mail: wangsiw@fmmu.edu.cn
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[1] Apostoli P. Fresenius’ J. Anal. Chem.，1999，363(5～6):499.
[2] Flerence T M. Talanta, 1982，29(5):345.
[3] WU Yi-wei, ZHANG Han，HU Bin，et al(吴一微，张 菡，胡 斌，等). Journal of Analysis Science(分析科学学报)，2003，19(3):201.
[4] CHEN Zhan-guo，DONG Tuan-rui，YUAN Yin-juan(陈战国，董团瑞，袁茵娟). China Journal of Chinese Materia Medica(中国中药杂志)，2005，30(21):1708.
[5] XIA Jian-qiang，ZHANG Ming-kui，FU Juan-lin(夏建强，章明奎，符娟林). Chinese Bulletin of Science and Technology(科技通报)，2006，22(4):482.
[6] PENG Shan，HUANG Guo-qing(彭 珊，黄国清). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005，25(2):296.
[7] FAN Wen-xiu(范文秀). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2007，27(7):1433.
[8] LIU Chang-zhan，LI Ai-hong(刘长占，李爱红). Studies of Trace Elements and Health(微量元素与健康研究)，2004，21(4): 24.
[9] CAO Zhi-quan, SUN Zuo-min, SUN Ai-zhen, et al(曹治权，孙作民，孙爱贞). Trace Elements and Traditional Chinese Medicine(微量元素与中医药). Beijing:China Traditional Chinese Medicine Press(北京:中国中医药出版社)，1993. 154.
[10] ZHOU Tian-ze(周天泽). Chinese Traditional Herbal Drugs(中草药)，1990，21(10):37.
[11] LI Shu-lan，LUO Xue-fang，MA Dan(李书兰，骆雪芳，马 丹). Advancement of Pharmaceutical Sciences(药学进展)，2006，30(2):49.
[12] TU Jian-bo，WANG Si-wang，XIE Yan-hua, et al(涂剑波, 王四旺, 谢艳华, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2007，27(11):2357.
[13] LIU Xiong，GAO Jian-de(刘 雄，高建德). Journal of Gansu Traditional Chinese Medical College(甘肃中医学院学报)，2007，24(2):46.
[14] SUN Li-li，FAN Xi-ying，ZHANG Dong-hong(孙丽莉，范锡英，张冬红). Trace Elements and Health Study(微量元素与健康研究)，2005，22(1):68.
[15] YUAN Bo-yong，YUAN Hui(袁伯勇，袁 慧). Studies of Trace Elements and Health(微量元素与健康研究)，1998，15(4):40.
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