| 光谱学与光谱分析 |
|
|
|
|
|
| Three-Dimensional Excitation Emission Matrix Fluorescence Spectroscopic Characterization of Loosely Bound and Tightly Bound Extracellular Polymeric Substances of Sludge |
| OUYANG Er-ming1, 2, WANG Wei1, LONG Neng3, LI Huai4 |
1. Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China
2. School of Civil Engineering and Architecture, Nanchang University, Nanchang 330031, China
3. School of Civil and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China
4. Department of Environmental Science and Engineering, Chang’an University, Xi’an 710054，China |
|
|
|
|
Abstract Three-dimensional excitation emission matrix fluorescence spectroscopy (3DEEM)was applied to characterize the loosely bound extracellular polymeric substances (LB-EPS)and tightly bound extracellular polymeric substances (TB-EPS)extracted from aerobic sludge in wastewater treatment. The results showed that three fluorescence peaks were identified in three-dimensional excitation emission matrix fluorescence spectra of the LB-EPS and TB-EPS: peak B (λex/λem=270-280 nm/345-360 nm), peak C (λex/λem=330-340 nm/410-430 nm)and peak D (λex/λem=390 nm/450-470 nm). The peak B was attributed to the protein-like fluorophores, peak C to the visible fulvic-like fluorophore and peak D to the humic-like fluorophore. Results of the peak intensity showed that the major component of the LB-EPS and TB-EPS is protein-like organic matter, then followed by (from high to low): fulvic-like organic matter and humic-like organic matter. The effects of both pH and concentration were significant to the 3DEEMs of LB-EPS and TB-EPS, but the degree is not the same. LB-EPS has the particular chemical structures which are different from the TB-EPS.
|
|
Received: 2008-03-02
Accepted: 2008-06-06
|
|
|
|
Corresponding Authors:
OUYANG Er-ming
E-mail: oyem02@mails.tsinghua.edu.cn;youmer@sina.com
|
|
[1] Houghton J I, Stephenson T. Water Research, 2002, 36(14): 3620.
[2] Frolund B, Palmgren R, Keiding K, et al. Water Research, 1996, 30(8): 1749.
[3] Wang Zhiping, Liu Lili, Yao Jie, et al. Chemosphere, 2006, 63(10): 1728.
[4] Liu H, Fang H H P. Journal of Biotechnology, 2002, 95(3): 249.
[5] WANG Hong-wu, LI Xiao-yan, ZHAO Qing-xiang(王红武，李晓岩，赵庆祥). China Safety Science Journal(中国安全科学学报)，2003，13(9):31.
[6] Li X Y, Yang S F. Water Research, 2007, 41(5): 1022.
[7] Wilen B M, Jin B, Lant P. Water Research, 2003, 37(9): 2127.
[8] Esparza-Soto M, Westerhoff P K. Water Science & Technology, 2001, 43(6): 87.
[9] SONG Ji-mei, TANG Bi-lian(宋继梅，唐碧莲). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2000, 20(1): 115.
[10] ZHANG Qian-qian, LEI Shu-he, WANG Xiu-lin, et al(张前前，类淑河，王修林，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(10): 1227.
[11] FU Ping-qing, LIU Cong-qiang, WU Feng-chang(傅平青，刘丛强，吴丰昌). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(12): 2024.
[12] FU Ping-qing, WU Feng-chang, LIU Cong-qiang, et al(傅平青，吴丰昌，刘丛强，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(3): 471.
[13] OUYANG Er-ming, ZHANG Xi-hui, WANG Wei(欧阳二明，张锡辉，王 伟). Water Resources Protection(水资源保护), 2007, 23(3): 56.
[14] OUYANG Er-ming, ZHANG Xi-hui, WANG Wei(欧阳二明，张锡辉，王 伟). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(7): 1373.
[15] Patel-Sorrentino N, Mounier S, Benaim J Y. Water Research, 2002, 36(10): 2571.
[16] Baker A. Environmental Science & Technology, 2002, 36(7): 1377.
[17] Wolfe A P, Kausal S S, Fulton J R, et al. Environmental Science & Technology, 2002, 36(15): 3217.
[18] Leenheer J A, Croue J P. Environmental Science & Technology, 2003, 37(1): 19A.
[19] Baker A. Water Research, 2002, 36(1): 189.
[20] Wu F C, Tanoue E. Environmental Science & Technology, 2001, 35(18): 3646.
[21] Mobed J J, Hemmingsen S L, Autry J L, et al. Environmental Science & Technology, 1996, 30(10): 3061.
[22] Baker A. Environmental Science & Technology, 2001, 35(5): 948. |
| [1] |
LEI Hong-jun1, YANG Guang1, PAN Hong-wei1*, WANG Yi-fei1, YI Jun2, WANG Ke-ke2, WANG Guo-hao2, TONG Wen-bin1, SHI Li-li1. Influence of Hydrochemical Ions on Three-Dimensional Fluorescence
Spectrum of Dissolved Organic Matter in the Water Environment
and the Proposed Classification Pretreatment Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 134-140. |
| [2] |
GU Yi-lu1, 2，PEI Jing-cheng1, 2*，ZHANG Yu-hui1, 2，YIN Xi-yan1, 2，YU Min-da1, 2, LAI Xiao-jing1, 2. Gemological and Spectral Characterization of Yellowish Green Apatite From Mexico[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 181-187. |
| [3] |
SONG Yi-ming1, 2, SHEN Jian1, 2, LIU Chuan-yang1, 2, XIONG Qiu-ran1, 2, CHENG Cheng1, 2, CHAI Yi-di2, WANG Shi-feng2，WU Jing1, 2*. Fluorescence Quantum Yield and Fluorescence Lifetime of Indole, 3-Methylindole and L-Tryptophan[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3758-3762. |
| [4] |
YANG Ke-li1, 2, PENG Jiao-yu1, 2, DONG Ya-ping1, 2*, LIU Xin1, 2, LI Wu1, 3, LIU Hai-ning1, 3. Spectroscopic Characterization of Dissolved Organic Matter Isolated From Solar Pond[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3775-3780. |
| [5] |
XUE Fang-jia, YU Jie*, YIN Hang, XIA Qi-yu, SHI Jie-gen, HOU Di-bo, HUANG Ping-jie, ZHANG Guang-xin. A Time Series Double Threshold Method for Pollution Events Detection in Drinking Water Using Three-Dimensional Fluorescence Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3081-3088. |
| [6] |
JIA Yu-ge1, YANG Ming-xing1, 2*, YOU Bo-ya1, YU Ke-ye1. Gemological and Spectroscopic Identification Characteristics of Frozen Jelly-Filled Turquoise and Its Raw Material[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2974-2982. |
| [7] |
YANG Xin1, 2, XIA Min1, 2, YE Yin1, 2*, WANG Jing1, 2. Spatiotemporal Distribution Characteristics of Dissolved Organic Matter Spectrum in the Agricultural Watershed of Dianbu River[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2983-2988. |
| [8] |
ZHU Yan-ping1, CUI Chuan-jin1*, CHENG Peng-fei1, 2, PAN Jin-yan1, SU Hao1, 2, ZHANG Yi1. Measurement of Oil Pollutants by Three-Dimensional Fluorescence
Spectroscopy Combined With BP Neural Network and SWATLD[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2467-2475. |
| [9] |
QIU Cun-pu1, 2, TANG Xiao-xue2, WEN Xi-xian4, MA Xin-ling2, 3, XIA Ming-ming2, 3, LI Zhong-pei2, 3, WU Meng2, 3, LI Gui-long2, 3, LIU Kai2, 3, LIU Kai-li4, LIU Ming2, 3*. Effects of Calcium Salts on the Decomposition Process of Straw and the Characteristics of Three-Dimensional Excitation-Emission Matrices of the Dissolved Organic Matter in Decomposition Products[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2301-2307. |
| [10] |
SHI Chuan-qi1, LI Yan2, HU Yu3, YU Shao-peng1*, JIN Liang2, CHEN Mei-ru1. Fluorescence Spectral Characteristics of Soil Dissolved Organic Matter in the River Wetland of Northern Cold Region, China[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1517-1523. |
| [11] |
LI Yuan-jing1, 2, CHEN Cai-yun-fei1, 2, LI Li-ping1, 2*. Spectroscopy Study of γ-Ray Irradiated Gray Akoya Pearls[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(04): 1056-1062. |
| [12] |
LIU Xia-yan1, CAO Hao-xuan1, MIAO Chuang-he1, LI Li-jun2, ZHOU Hu1, LÜ Yi-zhong1*. Three-Dimensional Fluorescence Spectra of Dissolved Organic Matter in Fluvo-Aquic Soil Profile Under Long-Term Composting Treatment[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 674-684. |
| [13] |
LÜ Yang1, PEI Jing-cheng1*, ZHANG Yu-yang2. Chemical Composition and Spectra Characteristics of Hydrothermal Synthetic Sapphire[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(11): 3546-3551. |
| [14] |
ZHANG Yong-bin1, ZHU Dan-dan1, CHEN Ying1*, LIU Zhe1, DUAN Wei-liang1, LI Shao-hua2. Wavelength Selection Method of Algal Fluorescence Spectrum Based on Convex Point Extraction From Feature Region[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(10): 3031-3038. |
| [15] |
PAN Hong-wei, TONG Wen-bin, LEI Hong-jun*, YANG Guang, SHI Li-li. Spectral Analysis of the Effect of Organic Fertilizer Application on the
Evolution of Organic Matter and Nitrogen in Farmaland[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(10): 3116-3123. |
|
|
|
|
