| 光谱学与光谱分析 |
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| Study on Interaction between Sulfonylurea Herbicides and Catalase by Fluorescence Spectroscopy |
| YE Fa-bing1, 2, DONG Yuan-yan1*, ZHOU Peng2, MO Xiao-man2, HU Xian-wen1 |
| 1. Department of Chemistry, College of Science, Huazhong Agriculture University, Wuhan 430070, China2. Department of Chemistry, Huanggang Normal University, Huanggang 438000, China |
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Abstract The binding of Sulfonylurea herbicides to catalase in aqueous solution was studied using fluorescence spectroscopy. It was shown that herbicides have a strong ability to quench the catalase fluorescence mainly through a static quenching procedure. The binding constant K and the number of binding site n were calculated according to the fluorescence quenching results. For chlorsufuron, K=8.69×105 L·mol-1 and n=1.16; for metsufuron methyl, K=1.01×106 L·mol-1 and n=1.21; and for bensufuron methyl, K=3.52×103 L·mol-1, n=0.77. It is clear that the binding of metsufuron methyl with catalase is stronger than that of chlorsufuron, while the binding of chlorsufuron stronger than that of bensufuron methyl.
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Received: 2005-06-26
Accepted: 2005-09-08
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Corresponding Authors:
DONG Yuan-yan
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| Cite this article: |
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YE Fa-bing,DONG Yuan-yan,ZHOU Peng, et al. Study on Interaction between Sulfonylurea Herbicides and Catalase by Fluorescence Spectroscopy [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(09): 1685-1687.
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| URL: |
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http://www.gpxygpfx.com/EN/Y2006/V26/I09/1685 |
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