| 光谱学与光谱分析 |
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| Spectroscopic Studies on the Binding of Prulifloxacin and ct-DNA |
| CHEN Chang-yun1，3, MA Mei-hua1, ZHOU Zhi-hua2, LU Kang1, XIANG Bing-ren3 |
1. Department of Chemistry, Nanjing Xiaozhuang College, Nanjing 210017, China
2. Department of Chemistry, Nanjing Normal University, Nanjing 210097, China
3. Analysis Center, China Pharmaceutical University， Nanjing 210009, China |
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Abstract The interaction mechanism of prulifloxacin(PL) and calf thymus DNA(ct-DNA) was studied by UV spectra, fluorescence spectra, and hydrodynamic measurements. The binding of ct-DNA and different concentrations of PL was discussed with UV, FL, phosphate effect and ion strength. The denaturation temperature and viscosity were measured. It is obvious that there is a hypochromic effect on the UV spectra with the fluorescence intensity decreasing regularly after addition of DNA and a red shift of the maximum emission peak with a static quenching constant of 3.1×104 L·mol-1. The above results show that a binary complex forms between PL and DNA. It is indicated that there is no static-electro interaction between them by phosphate effect. The outer groove binding is suggested by denaturation temperature of DNA increasing (no more than 7 ℃) and viscosity slightly decreasing.
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Received: 2005-06-01
Accepted: 2005-09-02
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Corresponding Authors:
CHEN Chang-yun
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| Cite this article: |
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CHEN Chang-yun,MA Mei-hua,ZHOU Zhi-hua, et al. Spectroscopic Studies on the Binding of Prulifloxacin and ct-DNA[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(10): 1891-1894.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2006/V26/I10/1891 |
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