| 光谱学与光谱分析 |
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| Study on Structures of Blattela Germanica Allergen, Bla g 2 Expressed by Eukaryotic and Prokaryotic Vectors Using Fluorescence and CD Spectra |
| LIU Zhi-gang1, ZHU Jian-qi1, HUANG Hai-zhen2, XU Hong1 |
1. College of Life Science, Shenzhen University, Shenzhen 518060, China
2. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China |
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Abstract The recombinant allergen, Bla g 2, was expressed by prokaryotic vector E.coli and eukaryotic vector P.Pastoris. The different structures and configurations of the Bla g 2 from E.coli and P.Pastoris were studied by fluorescence and circular dichroism. The secondary structures of Bla g 2 in solution, and the composition besides the type of its tertiary structure were proposed. These studies help understand the differences between prokaryotic and eukaryotic expression systems, reveal the relationship between the structure and the function of Bla g 2, and improve the production of this significant allergen.
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Received: 2005-11-28
Accepted: 2006-02-18
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Corresponding Authors:
LIU Zhi-gang
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| Cite this article: |
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LIU Zhi-gang,ZHU Jian-qi,HUANG Hai-zhen, et al. Study on Structures of Blattela Germanica Allergen, Bla g 2 Expressed by Eukaryotic and Prokaryotic Vectors Using Fluorescence and CD Spectra[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(05): 879-883.
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https://www.gpxygpfx.com/EN/Y2006/V26/I05/879 |
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