| 光谱学与光谱分析 |
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| Mechanism of Damage of DNA Induced by Carbaryl and Heavy Metal Ions |
| LIU Li-shuan,WANG Xin,YANG Bao-yuan,SUN Ying* |
| Key Laboratory of Pesticide Chemistry & Application Technology of Ministry of Agriculture, Department of Applied Chemistry, China Agricultural University, Beijing 100094, China |
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Abstract The effect of heavy metals on the adduct of carbaryl and calf thymus DNA in vitro was studied using fluorescence and UV spectrum. The results showed that heavy metals can influence the carbaryl quenching constant and the number of binding sites, and the order of is Pb2+>Ag2+>Cu2+>Ni2+>Co2+>Zn2+>Cd2+>Cr3+. The UV spectrum study showed that the heavy metal ions can change the double helix structure of ctDNA, and the order of this ability is Pb2+>Cd2+>Ag+>Co2+ >Zn2+≈Cu2+>Ni2+>Cr2+.
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Received: 2006-05-10
Accepted: 2006-08-20
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Corresponding Authors:
SUN Ying
E-mail: sunying@cau.edu.cn
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