| 光谱学与光谱分析 |
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| Effect of Acidity on the Interaction of Oflxacin and Bovine Serum Albumin |
| TANG Zhen-qiang1,HE Gan-wu2,YI Ping-gui1* |
1. School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Molecular Structure-ActivityRelationship Key Lab Hunan Province University, Xiangtan 411201, China
2. Shaoyang University, Shaoyang 422004, China |
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Abstract Bovine serum albumin (BSA) exists as N(pH -7.0), B(pH -9.0), and E (pH＜3.5)＝ isomeric forms in the solution of different pH. Acid effect on the structure of bovine serum albumin and the interaction of different structure of BSA with Oflxacin were studied by UV-Vis and fluorescence spectroscopy. Based on the fluorescence quenching of bovine serum albumin and Frster energy transfer mechanism, the quenching constants, energy transfer efficiencies and the binding distances were determined at four different pHs. The results showed that Oflxacin has the ability to quench bovine serum albumin fluorescence with the optimal condition of fluorescence quenching constants of 1.928 1×105 L·mo·l-1, binding distance of r=2.55 nm and quenching efficiency of 8.63×104 L·mo·l-1 at pH 4.9. Non-radiative energy transfer and static quenching were the cause of fluorescence quenching. The influence on the binding of Oflxacin and bovine serum albumin under neutral, subacidity and alkalescent conditions was not obviously observed, and the electrostatic interaction was not the main force. The effect of Oflx on the conformation of BSA was also investigated using synchronous fluorescence spectrometry.
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Received: 2006-12-11
Accepted: 2007-03-16
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Corresponding Authors:
YI Ping-gui
E-mail: yipinggui@sohu.com
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[1] Skauge T, Turel I, Sletten Einar. Inorganica Chimica Acta, 2002, 339: 239.
[2] Trynda-Lemiesz L, Keppler B K, Kozlowski H. J. Inorg Biochem, 1999, 73(3): 123.
[3] Charies B B, Hudson B S. J. Biological Chem., 1979, 254: 391.
[4] Dockal M, Carter D C, Ruker F. J. Biological Chem., 2000, 275: 3042.
[5] White D D, Sheena Stewart, Wood G C. The Federation of European Biochemical Sciences(FEBS), Letters, 1973, 33: 305.
[6] Tanaka N, Nishizawa H, Kunugi S. Biochim. Biophys. Acta, 1997, 1338: 13.
[7] Harmsen B J M, De Bruin S H, Janssen J H M. Biochemistry, 1971, 10: 3217.
[8] Basir Ahmad, et al. Archives of Biochem. Biophys., 2005, 437: 159.
[9] Octaaf J M Bos, Marcel J E Fischer, Jaap Wilting. Biochimica et Biophysica Acta, 1988, 953: 37.
[10] Basir Ahmad, et al. Biomacromoleculars, 2006, 7: 1350.
[11] Eftink M R, Ghiron C A. Anal. Biochem., 1981, 114: 199.
[12] YI Ping-gui, SHANG Zhi-cai, YU Qing-sen, et al(易平贵, 商志才, 俞庆森, 等). Acta Chimica Sinica（化学学报), 2000, 58(12): 1649.
[13] Johansson J S. J. Biol. Chem. 1997, 272: 17961.
[14] YI Ping-gui, SHANG Zhi-cai, YU Qing-sen, et al(易平贵, 商志才, 俞庆森, 等). Acta Chimica Physical Sinca(化学物理学报), 2003, 16: 420.
[15] WEI Xiao-fang, DING Xi-ming, LIU Hui-zhou(魏晓芳, 丁西明, 刘会洲). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2000, 20: 556.
[16] YANG Man-man, YANG Pin, XI Xiao-li(杨曼曼, 杨 频, 席小莉). Chinese Science Bulletin(科学通报), 2005, 20：2199.
[17] QIN Shen-jun, WEI Yong-ju(秦身钧, 魏永巨). Journal of the Chinese Rare Earth Society(中国稀土学报), 2004, 22: 393.
[18] NIE Li-hua, ZHAO Hui-chun, WANG Xue-bin(聂丽华, 赵慧春, 王学斌). Journal of Beijing Normal University·Natural Science(北京师范大学学报·自然科学版), 2001, 37: 87.
[19] YANG Man-man, YANG Pin, XI Xiao-li(杨曼曼, 杨 频, 席小莉). Chemical Journal of Chinese Universities(高等学校化学学报), 2006, 27: 687.
[20] LIU Cui-ge, XU Yi-zhuang, WEI Yong-ju(刘翠格, 徐怡庄, 魏永巨). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25: 584.
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