| 光谱学与光谱分析 |
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| Study of the Interaction Mechanism between Brodifacoum and DNA by Spectroscopy |
| DUAN Yun-qing1,2,MIN Shun-geng1* |
1. College of Science, China Agricultural University, Beijing 100094, China
2. College of Art and Science, Shanxi Agricultural University, Taigu 030801, China |
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Abstract The interaction between brodifacoum (3-[3-(4′-bromophenyl-4)1,2,3,4-tetralin-10]-4-hydroxyl-coumarin) (BDF), an anticoagulant rodenticide, and calf thymus DNA (ct-DNA) was studied by UV spectrum and fluorescence spectrum. The results were summarized as follows: There was a hypochromic effect of low concentration ct-DNA on the UV spectra. The fluorescence quenching studies showed a regular decrease in the fluorescence intensity after addition of ct-DNA by the static quenching mode with a quenching constant (KSV) of 1.21×104 L·mol-1 at 27 ℃. The BDF possibly bonded to ct-DNA mainly via Van der Waals forces by the corresponding thermodynamics parameter. KI quenching experiment found that there was not obvious protection of ct-DNA to BDF. The fluorescence intensity of BDF/ct-DNA system changed with the variation in ionic strength. Quenching of ct-DNA on the fluorescence of BDF/β-CD inclusion complex was reduced in contrast with the free BDF, which showed that β-CD could provide BDF with protection. So the comprehensive interaction mode of BDF with ct-DNA may be the groove binding by the above results. It was indicated that there had been static-electro interaction between BDF and ct-DNA at the same time. The conjunct action of Van der Waals forces and electrostatic attraction favorably provide BDF bonding interaction in the groove of ct-DNA.
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Received: 2007-11-16
Accepted: 2008-02-20
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Corresponding Authors:
MIN Shun-geng
E-mail: minsg@263.net
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