光谱学与光谱分析 |
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Spectroscopic Study on Interaction of Rodenticide Brodifacoum with Bovine Serum Albumin |
DUAN Yun-qing1, 2, LEI Huan-gui1, MIN Shun-geng2*, DUAN Zhi-qing3 |
1. College of Art and Science, Shanxi Agricultural University, Taigu 030801, China 2. College of Science, China Agricultural University, Beijing 100094, China 3. Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming 650118, China |
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Abstract The mutual interaction of bovine serum albumin (BSA) with brodifacoum (3-[3-(4’-bromophenyl-4)1,2,3,4-tetralin-10]-4-hydroxyl-coumarin), an anticoagulant rodenticide, was investigated by ultra-violet spectroscopy, flurorescence spectroscopy and synchronous fluorescence spectroscopy under physiological conditions. It was proved that the intrinsic fluorescence quenching of BSA by brodifacoum was the result of the formation of brodifacoum-BSA complex. And this quenching is mainly due to static fluorescence quenching. The quenching rate constant (KSV), binding site number (n) and binding constant (KA) at different temperatures were calculated from the double reciprocal Lineweaver-Burk plots and the quenching function of lg[(F0-F)/F]-lg[Q] plots. The thermodynamic parameters indicated that the process of binding was a spontaneous molecular interaction and the hydrophobic force played a major role in stabilizing the brodifacoum BSA complex. The binding distance r between brodifacoum and BSA was 2.84 and 2.87 nm at 20 and 30 ℃, respectively, which was obtained based on Forster theory of non-radiation energy transfer. The synchronous spectroscopy of BSA and brodifacoum-BSA revealed that the BSA conformation had changed in the presence of brodifacoum. The binding mode and interaction mechanism were suggested as follows: brodifacoum molecules are closed with amino acid residues with electric charge on the hydrophobic cavities of BSA by electrostatic interaction, and binded to the Trp212 residues inside of BSA hydrophobic cavities by hydrophobic interaction force, thereby changed the microenvironment around the Trp residues. The interaction prevented the energy transfer between Tyr and Trp residues, moreover, caused to a non-radiation energy transfer from Trp residues in BSA to brodifacoum, and finally leaded of the quenching the intrinsic fluorescence of BSA.
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Received: 2008-10-10
Accepted: 2009-01-20
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Corresponding Authors:
MIN Shun-geng
E-mail: minsg@263.net
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[1] YAN Cheng-nong, ZHANG Hua-xin, LIU Yi, et al(颜承农, 张华新, 刘 义, 等). Acta Chimica Sinica(化学学报), 2005, 63(18): 1727. [2] Pieter S, Van Heerden, Barend C B. Tetrahedron, 1997, 53(17): 6045. [3] Maurice R E, Camillo A G. Anal. Biochem., 1981, 114: 199. [4] XU Jin-gou, WANG Zun-ben(许金钩, 王尊本). Fluorescene Analysis Method(荧光分析法). Beijing: Science Press(北京:科学出版社), 2006. 64. [5] HU Yan-jun, LIU Yi, WANG Jia-bo, et al. J. Pharm. Biomed. Anal., 2004, 36(4): 915. [6] Sklar L A, Hudson B S, Simoni R D. Biochemistry, 1977, 16: 5100. [7] Majoul I, Straub M, Duden R, et al. Rev. Mol. Biotech., 2002, 82: 267. [8] Ross P D, Subramanian S. Biochem. 1981, 20 (11): 3096. [9] Wang Y Q, Zhang H M, Zhang G C, et al. J. Mol. Struc., 2006, 830: 40. [10] Burstein E A, Vedenkina N S. Irkova M N. Photochem. Photobio., 1973, 18: 263. [11] ZHU Keng, TONG Shen-yang(朱 铿, 童沈阳). Chem. J. Chin. Univ.(高等学校化学学报), 1996, 17(4): 539. [12] Shobini J, Mishra A K, Sandhya K, et al. Spectrochimica Acta Part A, 2001, 57: 1133. [13] Ulrich K H. J. Pharmacol Rev., 1981, 33: 17. |
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