| 光谱学与光谱分析 |
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| Study of Autofluorescence Spectra of Protoporphyrin Ⅸ in Human Serum |
| GUO Xing-jia1,2,LI Xiao-zhou3,XU Shu-kun1*,TONG Jian2,JIANG Yu-chun2,KANG Ping-li2 |
1. Chemistry Department of Northeast University, Shenyang 110004, China
2. College of Chemistry, Liaoning University, Shenyang 110036, China
3. Laser Biomedical Research Center, Shenyang Ligong University, Shenyang 110016, China |
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Abstract The fluorescence spectra of protoporphyrin Ⅸ (PPⅨ) solutions and human serum samples were measured and analyzed under physiological conditions. The experimental results showed that the fluorescence of PP Ⅸ in human serum was mainly derived from PPⅨ-serum albumin complex. Moreover, the effects of serum albumin and PPⅨ on the PP Ⅸ emission fluorescence were also investigated. Compared with pure PPⅨ solution, not only a red-shift of PPⅨ fluorescence peak was found for PPⅨ+albumin solution, but also albumin had a fluorescence enhancement effect on protoporphyrin Ⅸ. For the mixture of PPⅨ+albumin, with the increase of PPⅨ the wavelength of the PPⅨ emission peak will increased a little when its concentration was less than 0.8×10-5 mol·L-1,but was nearly invariable when its concentration was more than 0.8×10-5 mol·L-1.
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Received: 2006-01-10
Accepted: 2006-04-20
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Corresponding Authors:
XU Shu-kun
E-mail: xushukun46@126.com
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| Cite this article: |
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GUO Xing-jia,LI Xiao-zhou,XU Shu-kun, et al. Study of Autofluorescence Spectra of Protoporphyrin Ⅸ in Human Serum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(05): 995-998.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2007/V27/I05/995 |
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