|
|
|
|
|
|
Non-Destructive Detection of Male and Female Information of Early Duck Embryos Based on Visible/Near Infrared Spectroscopy and Deep Learning |
LI Qing-xu1, WANG Qiao-hua1, 2*, MA Mei-hu3, XIAO Shi-jie1, SHI Hang1 |
1. College of Engineering, Huazhong Agricultural University, Wuhan 430070, China
2. Key Laboratory of Agricultural Equipment in Mid-Lower Yangtze River,Ministry of Agriculture and Rural Agriculture, Wuhan 430070, China
3. National Egg Research and Development Center, Wuhan 430070, China |
|
|
Abstract Gender identification of embryonated eggs in China has always been a key issue in poultry industry development. In poultry meat production, males tend to be bred, while the egg production industry tends to breed females. If the male and female eggs can be identified in the early hatching process, it will reduce the cost of poultry hatching industry improve the economic benefits of poultry egg and meat production industry. This paper takes duck eggs as the research object. To realize the gender identification of duck eggs at the early hatching stage, a visible/near-infrared transmission spectrum acquisition system was constructed, which can collect the Spectral data of 345 duck eggs hatching from 0 to 8 days with the wavelength range of 200~1 100 nm. A 6-layer Convolutional Neural Network (CNN) for duck eggs’ spectral information was established, including input layer, 3 convolutional layers, 1 fully connection layer and output classification layer. The convolutional layer is used for extraction for the effective information in the spectrum. The full connected layer can integrate the local features extracted by the convolution layer for the classification decision of the output layer. In addition, the introduction of local response normalization and dropout operations in the convolutional neural network can accelerate the convergence speed of the neural network. The convolutional neural network was used to construct a duck embryo male and female information recognition network. By comparing and analyzing the recognition effects of different incubation days, it was found that the recognition effect was the best after 7 days of incubation. Subsequently, the duck eggs’ original spectral data hatched for 7 days were removed for noise, and the 500~900 nm band was selected for subsequent characteristic wavelength selection and modeling. Competitive adaptive reweighting algorithm (CARS), successive projections algorithm (SPA) and genetic algorithm (GA) were used to select the characteristic wavelengths that can distinguish the sex of duck embryos, and the selected characteristic wavelengths are converted into a two-dimensional spectral information matrix. The two-dimensional spectral information matrix retains the effective information of the one-dimensional spectrum and greatly facilitates the combination with the convolutional neural network. They were using a two-dimensional spectral information matrix combined with a convolutional neural network to achieve male and female identification of early hatching duck embryos. After testing, the model based on the SPA algorithm and the CNN network has a better effect, among the accuracy of the training set, development set, and test set are 93.36%, 93.12%, and 93.83%, respectively; the model based on the GA algorithm and CNN network was followed. In other words, the accuracy of the training set, development set and test set are 90.87%, 93.12%, and 86.42%, respectively; the accuracy of the training set, development set and a test set of the model based on the CARS algorithm and CNN network is 84.65%, 83.75%, 77.78%. The research results show that the visible/near-infrared spectroscopy technology and convolutional neural network can realize non-destructive identification of male and female duck embryos in early hatching, which provides technical support for developing subsequent related automated detection devices.
|
Received: 2020-06-14
Accepted: 2020-09-30
|
|
Corresponding Authors:
WANG Qiao-hua
E-mail: wqh@mail.hzau.edu.cn
|
|
[1] TANG Jian-lin,ZHOU Yu-lan(唐剑林,周玉兰). Guizhou Animal Science and Veterinary Medicine(贵州畜牧兽医), 2001,25(5):29.
[2] ZHU Zhi-hui, TANG Yong, HONG Qi, et al(祝志慧, 汤 勇, 洪 琪, 等). Transactions of the Chinese Society of Agricultural Engineering(农业工程学报), 2018, 34(6): 197.
[3] PAN Lei-qing, ZHANG Wei, YU Min-li, et al(潘磊庆, 张 伟,于敏莉,等). Transactions of the Chinese Society of Agricultural Engineering(农业工程学报), 2016, 32(1): 181.
[4] ZHU Zhi-hui, HONG Qi, WU Lin-feng, et al( 祝志慧, 洪 琪, 吴林峰, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2019, 39(9): 2780.
[5] Weissmann A, Reitemeier S, Hahn A, et al. Theriogenology, 2013, 80(3): 199.
[6] Turkyilmaz M K, Karagenc L, Fidan E. British Poultry Science, 2010, 51(4): 525.
[7] Galli R, Preusse G, Schnabel C, et al. PLOS ONE, 2018, 13: e0192554.
[8] Galli R, Preusse G, Uckermann O, et al. Analytical & Bioanalytical Chemistry, 2017, 409: 1185.
[9] Hirst C E, Major A T, Smith C A. International Journal of Developmental Biology, 2018, 62: 153.
[10] GAO Sheng, WANG Qiao-hua, LI Qing-xu, et al(高 升, 王巧华, 李庆旭, 等). Chinese Journal of Analytical Chemistry(分析化学), 2019, 47(6): 941.
[11] GAO Sheng, WANG Qiao-hua, FU Dan-dan, et al(高 升, 王巧华, 付丹丹,等). Acta Optica Sinica(光学学报), 2019,39(10): 1030004.
[12] WU Xi-yu(吴习宇). Doctoral Dissertation(博士论文). Southwest University(西南大学), 2018. |
[1] |
LIU Yan-de, WANG Shun. Research on Non-Destructive Testing of Navel Orange Shelf Life Imaging Based on Hyperspectral Image and Spectrum Fusion[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(06): 1792-1797. |
[2] |
JI Jiang-tao1, 2, LI Peng-ge1, JIN Xin1, 2*, MA Hao1, 2, LI Ming-yong1. Study on Quantitative Detection of Tomato Seedling Robustness
in Spring Seedling Transplanting Period Based on VIS-NIR
Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(06): 1741-1748. |
[3] |
PENG Ren-miao1, 2, XU Peng-peng2, ZHAO Yi-mo2, BAO Li-jun1, LI Cheng2*. Identification of Two-Dimensional Material Nanosheets Based on Deep Neural Network and Hyperspectral Microscopy Images[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(06): 1965-1973. |
[4] |
JIANG Rong-chang1, 2, GU Ming-sheng2, ZHAO Qing-he1, LI Xin-ran1, SHEN Jing-xin1, 3, SU Zhong-bin1*. Identification of Pesticide Residue Types in Chinese Cabbage Based on Hyperspectral and Convolutional Neural Network[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(05): 1385-1392. |
[5] |
JI Rong-hua1, 2, ZHAO Ying-ying2, LI Min-zan2, ZHENG Li-hua2*. Research on Prediction Model of Soil Nitrogen Content Based on
Encoder-CNN[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(05): 1372-1377. |
[6] |
ZHAO Yong1, HE Men-yuan1, WANG Bo-lin2, ZHAO Rong2, MENG Zong1*. Classification of Mycoplasma Pneumoniae Strains Based on
One-Dimensional Convolutional Neural Network and
Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(05): 1439-1444. |
[7] |
WENG Shi-zhuang*, CHU Zhao-jie, WANG Man-qin, WANG Nian. Reflectance Spectroscopy for Accurate and Fast Analysis of Saturated
Fatty Acid of Edible Oil Using Spectroscopy-Based 2D Convolution
Regression Network[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(05): 1490-1496. |
[8] |
ZHANG Xiao-hong1, JIANG Xue-song1*, SHEN Fei2*, JIANG Hong-zhe1, ZHOU Hong-ping1, HE Xue-ming2, JIANG Dian-cheng1, ZHANG Yi3. Design of Portable Flour Quality Safety Detector Based on Diffuse
Transmission Near-Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(04): 1235-1242. |
[9] |
XIAO Shi-jie1, WANG Qiao-hua1, 2*, LI Chun-fang3, 4, DU Chao3, ZHOU Zeng-po4, LIANG Sheng-chao4, ZHANG Shu-jun3*. Nondestructive Testing and Grading of Milk Quality Based on Fourier Transform Mid-Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(04): 1243-1249. |
[10] |
LI Lian-jie1, 2, FAN Shu-xiang2, WANG Xue-wen1, LI Rui1, WEN Xiao1, WANG Lu-yao1, LI Bo1*. Classification Method of Coal and Gangue Based on Hyperspectral
Imaging Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(04): 1250-1256. |
[11] |
TAN Ai-ling1, CHU Zhen-yuan1, WANG Xiao-si1, ZHAO Yong2*. Detection of Pearl Powder Adulteration Based on Raman Spectroscopy and DCGAN Data Enhancement[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(03): 769-775. |
[12] |
ZHANG Fu1, 2, 3, CUI Xia-hua1, DING Ke4*, ZHANG Ya-kun1, WANG Yong-xian1, PAN Xiao-qing5. Study on the Influence of Different Pretreatment Methods on Gender Determination of Multiposition[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(02): 434-439. |
[13] |
DENG Shi-yu1, 2, LIU Cheng-zhi1, 4*, TAN Yong3*, LIU De-long1, ZHANG Nan1, KANG Zhe1, LI Zhen-wei1, FAN Cun-bo1, 4, JIANG Chun-xu3, LÜ Zhong3. A Combination of Multiple Deep Learning Methods Applied to Small-Sample Space Objects Classification[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(02): 609-615. |
[14] |
JIAO Qing-liang1, LIU Ming1*, YU Kun2, LIU Zi-long2, 3, KONG Ling-qin1, HUI Mei1, DONG Li-quan1, ZHAO Yue-jin1. Spectral Pre-Processing Based on Convolutional Neural Network[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(01): 292-297. |
[15] |
ZHANG Jing1, 2, XU Yang1, JIANG Yan-wu1, ZHENG Cheng-yu2, ZHOU Jun1,2, HAN Chang-jie1*. Recent Advances in Application of Near-Infrared Spectroscopy for Quality Detections of Grapes and Grape Products[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(12): 3653-3659. |
|
|
|
|