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| Rapid Non-Destructive Detection Method for Black Tea With Exogenous Sucrose Based on Near-Infrared Spectroscopy |
| LUO Zheng-fei1, GONG Zheng-li1, 2, YANG Jian1, 2*, YANG Chong-shan2, 3, DONG Chun-wang3* |
1. School of Biotechnology and Engineering, West Yunnan Normal University of Science and Technology, Lincang 677000, China
2. School of Engineering and Technology, Southwest University, Chongqing 400715, China
3. Tea Research Institute, Shandong Academy of Agricultural Sciences，Jinan 250100，China
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Abstract In order to realize the rapid and effective detection of exogenous sucrose content in finished black tea, Fengqing large-leaved species tea was used as a research sample, and a quantitative prediction model for exogenous sucrose content in finished black tea was constructed by using near-infrared spectroscopy. First, near-infrared spectral data were collected during the production of finished black tea samples with different exogenous sucrose contents (0, 250, 500 and 750 g). When processing the data, in order to improve the prediction accuracy of the model, four different preprocessing methods, standard normal transformation (SNV), multivariate scattering correction (MSC), smoothing (Smooth) and centering (Center), were selected to reduce noise and establish partial least squares regression ( PLSR) model, according to the effect of the model, the best SNV preprocessing method was selected, the correction set correlation coefficient (Rc) was 0.907, the prediction set correlation coefficient (Rp) was 0.826, and the relative percent deviation (RPD) was 1.75. In order to reduce the impact of redundant information in the spectrum on the model operation speed, the competitive adaptive reweighted sampling (CARS), shuffled frog leaping algorithm(SFLA), variable combination population analysis iteratively retaining informative variables (VCPA-IRIV) and variable iterative space shrinkage algorithm (VISSA) to extract the characteristic wavelengths sensitive to sucrose from the SNV preprocessed spectrum. After the full spectrum and the selected characteristic wavelengths were dimensionally reduced by principal component analysis (PCA), linear PLSR and nonlinear support vector regression (SVR) and random forest (RF) quantitative prediction models were established respectively. The results show that after SNV preprocessing, the performance of the nonlinear SVR and RF models is better than that of the linear PLSR model, among which VCPA-IRIV-SVR is the optimal model, its Rc value is 0.950, Rp value is 0.924, and RPD value is 2.51. The research shows that near-infrared spectroscopy is feasible for the quantitative prediction of sucrose content in black tea processing, which provides a theoretical support for the non-destructive testing of black tea safety and quality.
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Received: 2022-04-07
Accepted: 2022-09-07
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Corresponding Authors:
LUO Zheng-fei1, GONG Zheng-li1, 2, YANG Jian1, 2*, YANG Chong-shan2, 3, DONG Chun-wang3*
E-mail: dongchunwang@163.com
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