| 光谱学与光谱分析 |
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| Effect of UV on the Absorption Spectra of E.coli Cells |
| YANG Tian-you1,2,LI Zong-wei1,ZHANG Feng-qiu1,CHEN Lin-hai1,LI Zong-yi1,QIN Guang-yong1*,HUO Yu-ping1 |
| 1. Provincial Key Laboratory of Ion Beam Bioengineering,Zhengzhou University,Zhengzhou 450052,China2. Life Science and Technology College,Henan Institute of Science and Technology,Xinxiang 453003，China |
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Abstract The present article studied the effects of different solutions in sample cavity on the spectra of E.coli. It is shown that the solutions of 2.8 mL distilled water and 0.1 mL LB and 0.1 mL PBS is the best,because it not only supplies good living condition but also has less effect on the spectra of E.coli. And it was found that UV in spectrophotometer has no effect on the mensuration result. On the basis of these studies the absorption spectra of E.coli irradiated by different doses UV were investigated. It is shown that there is a declining-rising-declining phenomenon in the spectra of E.coli with the dose increasing. This reflects the wound healing process of E.coli. Spectroscopic technique is helpful for the study of cell’s wound healing.
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Received: 2006-05-26
Accepted: 2006-08-28
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Corresponding Authors:
QIN Guang-yong
E-mail: yangtianyou2004@163.com
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| Cite this article: |
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YANG Tian-you,LI Zong-wei,ZHANG Feng-qiu, et al. Effect of UV on the Absorption Spectra of E.coli Cells[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(08): 1600-1602.
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| URL: |
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http://www.gpxygpfx.com/EN/Y2007/V27/I08/1600 |
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