Comparison of Enhancement Effect of DNA-Mediated Energy Transfer by Divalent Cations: Mg2+, Ca2+, Mn2+, Co2+, and Ni2+
Jong-Moon Kim1, Myung Duk Jang2, JIN Biao3*, Yoon Jung Jang4*
1. Department of Health Care and Biotechnology, Kyungwoon University, Gumi City, Gyeong-buk 730-739, Republic of Korea
2. Materials & Energy Engineering College of IT & Energy, Kyungwoon University, Gumi City, Gyeong-buk 730-739, Republic of Korea
3. Instrumental Analysis Center, Yanbian University, Yanji 133002, China
4. College of Basic Education, Yeungnam University, Dae-dong, Gyeongsan City, Gyeong-buk 712-749, Republic of Korea
Comparison of Enhancement Effect of DNA-Mediated Energy Transfer by Divalent Cations: Mg2+, Ca2+, Mn2+, Co2+, and Ni2+
Jong-Moon Kim1, Myung Duk Jang2, JIN Biao3*, Yoon Jung Jang4*
1. Department of Health Care and Biotechnology, Kyungwoon University, Gumi City, Gyeong-buk 730-739, Republic of Korea
2. Materials & Energy Engineering College of IT & Energy, Kyungwoon University, Gumi City, Gyeong-buk 730-739, Republic of Korea
3. Instrumental Analysis Center, Yanbian University, Yanji 133002, China
4. College of Basic Education, Yeungnam University, Dae-dong, Gyeongsan City, Gyeong-buk 712-749, Republic of Korea
摘要： The effect of various metal ions on the DNA mediated energy transfer between simultaneously bound drugs was investigated using spectroscopic methods. It was found that addition of divalent metal ions (Mg2+, Ca2+, Mn2+, Co2+ and Ni2+) resulted in further decrease of the ethidium fluorescence intensity, while a small increase was observed in the TMPyP emission band, implying that the energy of excited ethidium was transferred to TMPyP. This DNA-mediated quenching efficiency between ethidium and TMPyP was significantly enhanced by the presence of all metal ions. Among the divalent metal ions, alkali earth metal ions and Mn2+ displayed higher quenching efficiencies than other transition metal ions. The distances required to permit the energy transfer between the two drugs in DNA were calculated as 68, 66, 62, 48 and 38 in the presence of 100 μmol·L-1 of Mg2+, Ca2+, Mn2+, Co2+ and Ni2+ ion, respectively. The disturbed binding conformation of TMPyP in DNA by metal ions presumably accounts for the difference.
Abstract：The effect of various metal ions on the DNA mediated energy transfer between simultaneously bound drugs was investigated using spectroscopic methods. It was found that addition of divalent metal ions (Mg2+, Ca2+, Mn2+, Co2+ and Ni2+) resulted in further decrease of the ethidium fluorescence intensity, while a small increase was observed in the TMPyP emission band, implying that the energy of excited ethidium was transferred to TMPyP. This DNA-mediated quenching efficiency between ethidium and TMPyP was significantly enhanced by the presence of all metal ions. Among the divalent metal ions, alkali earth metal ions and Mn2+ displayed higher quenching efficiencies than other transition metal ions. The distances required to permit the energy transfer between the two drugs in DNA were calculated as 68, 66, 62, 48 and 38 in the presence of 100 μmol·L-1 of Mg2+, Ca2+, Mn2+, Co2+ and Ni2+ ion, respectively. The disturbed binding conformation of TMPyP in DNA by metal ions presumably accounts for the difference.
基金资助: the National Research Foundation of Korea (20011-0013534，2009-0076930) and the National Natural Science Foundation of China (21265022)
通讯作者:
JIN Biao, Yoon Jung Jang
E-mail: jinbiao@ybu.edu.cn; jyj5014@ynu.ac.kr
作者简介: e-mail:
jmkim@kw.ac.kr
引用本文:
Jong-Moon Kim, Myung Duk Jang, JIN Biao, Yoon Jung Jang. Comparison of Enhancement Effect of DNA-Mediated Energy Transfer by Divalent Cations: Mg2+, Ca2+, Mn2+, Co2+, and Ni2+[J]. 光谱学与光谱分析, 2018, 38(06): 1970-1974.
Jong-Moon Kim, Myung Duk Jang, JIN Biao, Yoon Jung Jang. Comparison of Enhancement Effect of DNA-Mediated Energy Transfer by Divalent Cations: Mg2+, Ca2+, Mn2+, Co2+, and Ni2+. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1970-1974.
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