Study on the Fluorescence Resonance Energy Transfer between CdTe QDs and Rhodamine B and Its Application to the Determination of Trace Amount of Copper in Flos Lonicerae Japonicae
WANG Xiao-mei1, YE Chao1, LI Chen-gui1, HU Yu-zhu1, 2*
1. Department of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China 2. Key Laboratory of Drug Quality Control and Pharmacovigilance Affiliated to Ministry of Education, China Pharmaceutical University, Nanjing 210009, China
Abstract：The present work is to find the optimum condition for fluorescence resonance energy transfer between CdTe QDs (donor) and rhodamine B (acceptor) and form a new method to detect copper in Flos Lonicerae Japonicae. In tris-HCl buffer solution at pH 6.00, by employing cetyltrimethylammonium bromide, Cu2+ can quench the fluorescence intensity of BRB and detect the concentration of Cu2+. Results show that a linear relationship could be established between the quenched fluorescence intensity of RhB and the concentration of Cu2+ in the range of 1.3×10-4～3.1×10-2 μg·mL-1, the limit of detection was 4.6×10-5 μg·mL-1 (r=0.996), RSD and the average recovery was 3.5% and 103.4% (n=5), respectively. It is concluded that the developed method was proved to be feasible to detect copper in Flos Lonicerae Japonicae.
王晓梅1, 叶 超1, 李臣贵1, 胡育筑1,2* . CdTe量子点-罗丹明B荧光共振能量转移猝灭法测定金银花中的微量铜 [J]. 光谱学与光谱分析, 2011, 31(02): 448-451.
WANG Xiao-mei1, YE Chao1, LI Chen-gui1, HU Yu-zhu1, 2* . Study on the Fluorescence Resonance Energy Transfer between CdTe QDs and Rhodamine B and Its Application to the Determination of Trace Amount of Copper in Flos Lonicerae Japonicae. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2011, 31(02): 448-451.
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