BAI Jin-wei1, CHI Yan-hua1*, ZHUANG Jia1, LI Ke-an2
1. College of Material Science and Engineering, Southwest University of Science and Technology, Mianyang 621002, China 2. College of Chemistry and Molecular Engineering, Peking University,Beijing 100871, China
Abstract:The interaction of lysozyme(Lys),O-{2-〔α-(2-Hydroxy-5-Sulphophenlylazo)-benzylidene〕hydrazino}benzoic acid(ZCN) and cetyltrimethy ammonicumbromide(CTMAB) was investigated by UV spectrophotometric method. The effect of temperature,time,and ion intensity in the buffer solution (pH 4.46) on reaction system was determined. Linear range of the reaction was 0~10 μg·mL-1. The molar absorptivity was ε=4.616 4×105 L·cm-1·mol-1. The reaction mechanism and interference of inorganic substance,biological substance and surface active agent to the reaction was investigated. Study system was established.
白进伟1,迟燕华1*,庄 稼1,李克安2 . 溶菌酶-ZCN-CTMAB三元配合物的反应机理研究 [J]. 光谱学与光谱分析, 2004, 24(09): 1082-1085.
BAI Jin-wei1, CHI Yan-hua1*, ZHUANG Jia1, LI Ke-an2 . Study on the Reaction of Lys-ZCN-CTMAB Complex . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(09): 1082-1085.
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