Abstract:The interaction of hesperidin (HES) or icariin (ICA) and lysozyme (LYS) was studied by fluorescence spectroscopy in physiological buffer solution. It was observed that there was a strong fluorescence quenching effect of hesperidin or icariin on lysozyme. The quenching constants of the drugs with lysozyme were measured at different temperatures, and the quenching mechanism was suggested as dynamic quenching for HES-LYS system and both static and dynamic quenching for ICA-LYS system. The thermodynamic parameters of the interaction of hesperidin or icariin and lysozyme were measured according to the Van’s Hoff equation: the enthalpy change (ΔH) and the entropy change (ΔS) of HES-LYS system and ICA-LYS system were calculated to be 20.29 kJ·mol-1 and 146.28 J·mol-1·K-1,and -3.47 kJ·mol-1 and 81.16 J·mol-1·K-1,respectively, which indicated that the interaction of hesperidin and lysozyme was driven mainly by hydrophobic force, whereas the interaction of icariin and lysozyme was driven mainly by electrostatic force. It was showed that the reaction processes of the two systems occurred spontaneously since Gibbs free energy change (ΔG) values were negative. The binding distances of hesperidin and icariin from the lysozyme tryptophan residue were calculated to be 1.34 nm and 1.24 nm, respectively, based on the Frster’s theory of non-radiation energy transfer. The results of synchronous fluorescence spectra showed that the binding of hesperidin or icariin to lysozyme induced conformational changes in lysozyme.
张国文,陈秀霞,郭金保,王俊杰. 荧光法研究橙皮苷、淫羊藿苷与溶菌酶的相互作用[J]. 光谱学与光谱分析, 2009, 29(01): 184-187.
ZHANG Guo-wen, CHEN Xiu-xia, GUO Jin-bao, WANG Jun-jie. Study on the Interaction of Hesperidin or Icariin with Lysozyme by Fluorescence Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(01): 184-187.
[1] YI Ping-gui, YU Qing-sen, SHANG Zhi-cai, et al(易平贵, 俞庆森, 商志才, 等). Acta Phamaceutica Sinica(药学学报), 2000, 35(10): 774. [2] Pyle A M, Morii T, Barton J K. J. Am. Chem. Soc., 1990, 112: 9432. [3] WANG Chun, WU Qiu-hua, WANG Zhi,et al(王 春, 吴秋华, 王 志, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007, 27(4): 754. [4] BIAN Wei, WEI Yan-li, WANG Ya-ping, et al(卞 伟, 卫艳丽, 王亚萍, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(3): 505. [5] Hu Y J, Liu Y, Zhang L X, et al. J. Mol. Struct., 2005, 750: 174. [6] He W Y, Li Y, Tang J H, et al. Int. J. Biol. Macromol., 2006, 39: 165. [7] Jeffrey S N, John J R, Bruce S Z, et al. Cancer Res., 1981, 4: 1642. [8] OU Ming, LIN Li, LI Yan-wen(欧 明, 林 励, 李衍文). Handbook of Concise Chinese Traditional Medicine Component(简明中药成分手册). Beijing: Chinese Medicine Science and Technology Press(北京: 中国医药科技出版社), 2003. 202. [9] QIN De-an, SU Dan, WANG Xiao-ling(秦德安, 苏 丹, 王晓玲). Chinese Pharmaceutical Journal(中国药学杂志), 1996, 31(7): 396. [10] Li W K, Zhang R Y, Xiao P G. Phytochemistry, 1996, 43: 527. [11] CHEN Guo-zhen, HUANG Xian-zhi, ZHENG Zhu-zi, et al (陈国珍, 黄贤智, 郑朱梓, 等). The Methods of Fluorescence Analysis, Second ed(荧光分析法·第2版). Beijing: Science Press(北京: 科学出版社), 1990. 112. [12] Lakowicz J R. Principles of Fluorescence Spectroscopy(Second ed.). New York:Plenum Press, 1999. 237. [13] Lakowicz V B, Kryvorotenko D V, Balanda A O, et al. Dyes and Pigments, 2005, 67: 47. [14] Ross D P, Subramanian S. Biochemistry, 1981, 20: 3096. [15] YANG Pin, GAO Fei, MA Gui-bin(杨 频, 高 飞, 马贵斌). An Introduetion of Bioinorganic Chemistry(生物无机化学导论). Xi’an: Xi’an Communications University Press(西安: 西安交通大学出版社), 1991. 152. [16] Lakowicz J R, Weber G. Biochemistry, 1973, 12: 4161. [17] Miller J N. Analytical Proceedings,1979, 16: 203. [18] Anna S J. Mol. Struct., 2002, 614: 227.