Study on the Reaction of Proteinase from Bacillus Subtilis with Alizarin Complex
WANG Xing-ming1，DONG Fa-qing1，DING Li-sheng2，YANG Ding-ming1，SHI Rong-ming1
1. Department of Chemistry and Biology Engineering，Southwest University of Science and Technology，Mianyang 621010, China 2. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
Abstract：The interaction of bacillus subtilis proteinase(BSP) and Alizarin Complex(ALC) was investigated by spectrophotometric method in acidic solution(pH 4.20). When BSP was added into ALC, a red color was observed, which indicated the formation of BSP-ALC associated compound. The maximum absorption of the red color coordination compound was obtained at 510 nm. It was found that the red shift of maximum absorption of the complex was 85 nm. According to balance percolation method, molar ratio method and double wavelengh method, the apparent molar absorptivity εB＝6.68×103 L·mol-1·cm-1. Conditional constants were defined, the maximum binding number n＝10, and the apparent binding constant K＝7.25×106 L·mol-1. It was found that Scatchard model is appropriate in the treatment of data obtained here.
王兴明1，董发勤1，丁立生2，杨定明1，石荣铭1 . 枯草杆菌蛋白酶与茜素氨羧络合剂作用研究[J]. 光谱学与光谱分析, 2005, 25(09): 1471-1474.
WANG Xing-ming1，DONG Fa-qing1，DING Li-sheng2，YANG Ding-ming1，SHI Rong-ming1 . Study on the Reaction of Proteinase from Bacillus Subtilis with Alizarin Complex . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(09): 1471-1474.
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