WANG Dong-dong1, 2,MENG Qing-xiang1,CHEN Dan-dan1,REN Li-ping1*,ZHOU Zhen-ming1,MU Hao-jie1
1. College of Animal Science/Beef Cattle Research Center, China Agricultural University, State Key Laboratory of Animal Nutrition, Beijing 100094, China 2. College of Science, North China University of Technology, Beijing 100041, China
Abstract：The fluorescence spectra, absorption spectra and resonance light scattering (RLS) spectra of EGCG-Cu(Ⅱ) complex with nucleic acid were studied. Compared with the spectrum of EGCG-Cu(Ⅱ), the spectrum of EGCG-Cu(Ⅱ)-DNA system showed the following features: (1) in the fluorescence spectra, the peak did not shift, but the fluorescence intensity was enhanced and was proportional to the concentration of DNA. Under suitable conditions, EGCG-Cu(Ⅱ) promised to be a probe for detecting DNA; (2) Absorption spectra of EGCG-Cu(Ⅱ)-DNA system showed hyperchromicity; and (3) RLS of EGCG-Cu(Ⅱ)-DNA system was enhanced also. A mechanism was suggested that the combination of nucleic acid and EGCG-Cu(Ⅱ) complex was caused by intercalation and electrostatic force. An intercalation interaction between EGCG-Cu(Ⅱ) and DNA enhanced the fluorescence intensity. Hyperchromicity of absorption spectra and RLS occurred because of electrostatic force. The effects of acid and ionic strength on fluorescence intensity were also discussed.
[1] YANG Pin, SONG Yu-fei(杨 频, 宋宇飞). Progress in Chemistry(化学进展), 2000, 12(1): 32. [2] Clement M V, Hirpara J L, Chawdhury S H, et al. Blood, 1998, 92: 996. [3] Azam S, Hadi N, Khan N U, et al. Toxicology in Vitro, 2004, 18: 555. [4] YANG Pei-ju, SUN Kun, SU Xue, et al(杨培菊, 孙 坤, 苏 雪, 等). Acta Chimica Sinica(化学学报), 2005, 63(23): 2153. [5] Yang Z S, Wang Y L, Zhang Y Z. Electrochemistry Communications, 2004, 6(2): 158. [6] YI Ping-gui, SHANG Zhi-cai, YU Qing-sen(易平贵,商志才, 俞庆森). Chinese Journal of Inorganic Chemistry(无机化学学报), 2001, 17(1): 77. [7] ZHANG Yu-yan, SHEN Sheng-rong, TANG De-song, etal(张玉艳, 沈生荣, 唐德松, 等). Journal of Zhejiang University(Agriculture and Life Science)(浙江大学学报·农业与生命科学版), 2004, 30(3): 290. [8] Pasternack R F, Bustamante C, Collings P J, et al. J. Am. Chem. Soc., 1993, 115: 5393. [9] CHEN Dan-dan, ZHANG Tao, REN Li-ping, et al(陈丹丹, 张 涛, 任丽萍, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006，26(14): 686. [10] SHI Deng-rong, ZHANG Tao, REN Li-ping, et al(石登荣, 张 涛, 任丽萍, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006, 26(13): 509. [11] WANG Ya-ting, ZHAO Feng-lin, LI Ke-an, et al(王亚婷, 赵凤林, 李克安, 等). Chemical Research in Chinese Universities(高等学校化学学报), 2000, 21(10): 1491. [12] Mahadevan S, Palaniandavar M. Inorganica Chimica Acta, 1997, 254(2): 291. [13] ZHANG Qian-ling, LIU Jian-hong, REN Xiang-zhong(张黔玲, 刘剑洪, 任祥忠, 等). Acta Chimica Sinica(化学学报), 2006, 64(10): 968. [14] Vijayalakshmi R, Kanthimathi M, Subramanian V, et al. Biochimica et Biophysica Acta, 2000, 1475(2): 157. [15] MA Chun-qi, LIU Ying, LI Ke-an, et al(马春琪, 刘 瑛, 李克安, 等). Chinese Science Bulletin(科学通报), 1999, 44(7): 682. [16] XI Xiao-li, YANG Man-man, HAN Xiao-jian, et al(席小莉, 杨曼曼, 韩小见, 等). Chinese Journal of Inorganic Chemistry(无机化学学报), 2001, 11(6): 781.
