FTIR Analysis of the Enzymatic Degradation of Chitosan Blend Membranes
REN Dong-wen1, WANG Yi-li1, BAO De-cai1, 2, XIE Wei-yang1, MA Xiao-jun1*
1. Lab of Biomedical Materials Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, and Graduate School of Chinese Academy of Sciences, Dalian 116023, China 2. College of Chemistry and Chemical Engineering, Bohai University, Jinzhou 121000, China
Abstract：Chitosan blend membranes were prepared by casting the mixture solution of highly deacetylated chitosan (HDC) and moderately deacetylated chitosan (MDC). FTIR was used to investigate the components of the blend membranes before and after lysozymic degradation. It was found that the ratio of MDC in the blend membranes had a linear relation with the degree of deacetylation(DD) of the membranes. In enzymatic degradation process, DD of the membranes exhibited an increasing tendency. The FTIR data indicate that MDC component in the blend membranes can be removed by selective enzymatic degradation. This also suggests that FTIR can be used as an efficient and rapid method to investigate the degradation process of chitosan.
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