Detection of Antioxidative Capacity of Bamboo Leaf Extract by Scavenging Hydroxyl Free Radical
GUO Xue-feng1, YUE Yong-de1*, MENG Zhi-fen2, TANG Feng1, WANG Jin1, YAO Xi1, XUN Hang1, SUN Jia1
1. State Forestry Administration Key Open Laboratory on Bamboo and Rattan Science and Technology, International Centre for Bamboo and Ratten, Beijing 100102, China 2. School of Chemistry and Chemical Engineering, Henan Institute of Science and Technology, Xinxiang 453003, China
Abstract：By studying on absorption spectrum of red compound coming from the reaction system of Fe2+ and 1,10-phenanthroline and the capacity of antioxidant TBHQ and bamboo leaf extract for scavenging hydroxyl free radical, some results were drawn as follows: the determining wavelength of bamboo leaf extract for scavenging hydroxyl free radical by spectrophotometric method is 509.1 nm, and IC50 (the value of antioxidant concentration at scavenging half of hydroxyl free radical)was used as the index to evaluate scavenging capacity. The determined IC50 values were TBHQ(0.040), M20(0.378), M40(0.323), M60(0.334), and bamboo leaf extract could be used as natural antioxidant.
Key words：Bamboo leaf extract;Hydroxyl free radical;Scavenging rate;IC50 value
