Quantitative Detection of Hydroxyl Radical Generated in Quartz Powder/Phosphate Buffer Solution System by Fluorescence Spectrophotometry
LIU Li-zhu1, 2, DONG Fa-qin2*, SUN Shi-yong2, HE Xiao-chun2, WANG Ming-cui3
1. School of Environment and Resource, Southwest University of Science and Technology, Mianyang 621010, China 2. Key Laboratory of Solid Waste Treatment and Resource Recycle of Ministry of Education, Mianyang 621010, China 3. School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
Abstract：Quartz powder would release radical hydroxyl in phosphate buffer solution. In order to detect the quantity of radical hydroxyl with a quite low concentration, the present paper established a fluorescence method. According to the relationship between the concentration of 2-hydroxyl of terephthalic acid (HOTP) and fluorescence intensity at the wavelength of EXmax/EMmax=316 nm/422 nm, a working standard curve was constructed. Then through the filtrated fluorescence intensity obtained from the powder and solution system, cumulative concentration of ·OH can be measured indirectly. By repeating tests and analysis of five different experimental conditions of ·OH cumulative concentration, the minimum detection limit of the method reached 1.59×10-10 mol·L-1, with a relative deviation of 1.20%~7.89%, standard deviation was 1.09×10-9~2.17×10-9 mol·L-1 and the relative standard deviation was 3.5%~5.8%. The method features high accuracy and good repeatability performance. Compared to other quantitative studies, this method might be applied to test radical hydroxy produced in pH neutral solution systems. In addition, it has apparent advantages such as low detection limit, low cost, higher sensitivity, and better stability and reproducibility. That provides the means for the quantitative study of mixed systems consisting of quartz powder and phosphate buffered solution.
刘立柱1，2，董发勤2*，孙仕勇2，贺小春2，王明翠3 . 石英粉/磷酸盐缓冲溶液体系中羟自由基荧光分光光度法定量检测研究 [J]. 光谱学与光谱分析, 2014, 34(07): 1886-1889.
LIU Li-zhu1, 2, DONG Fa-qin2*, SUN Shi-yong2, HE Xiao-chun2, WANG Ming-cui3 . Quantitative Detection of Hydroxyl Radical Generated in Quartz Powder/Phosphate Buffer Solution System by Fluorescence Spectrophotometry . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(07): 1886-1889.
[1] Vallyathan V. Environmental Health Perspectives, 1994, 102(Suppl. 10): 111. [2] CAO Yan-ping, YANG Ying-mao, ZHU Yu-chen(曹雁平，袁英髦，朱辰雨). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2012, 32(5): 1320. [3] Yang W, Deng Y. Chinese Journal of Analysis Laboratory, 2007, 26(9): 120. [4] Shimizu N, Ogino C, Dadjour M F, et al. Ultrason Sonochem., 2007, 14(2): 184. [5] Boulanouar B, Abdelaziz G, Aazza S, et al. Industrial Crops and Products, 2013, 46: 85. [6] Oliveira R, Bento F, Geraldo D. Journal of Electroanalytical Chemistry, 2012, 682(0): 7. [7] Zanta C, Martínez-Huitle C. Brazilian Journal of Chemical Engineering, 2009, 26(3): 503. [8] Li S, Zhao Y, Zhang L, et al. Food Chem., 2012, 135(3): 1914. [9] Berberidou C, Poulios I, Xekoukoulotakis N, et al. Applied Catalysis B: Environmental, 2007, 74(1): 63. [10] Zhang N D, Zheng W. Journal of Instrumental Analysis, 2002，(5): 36. [11] Aranda A, Sequedo L, Tolosa L, et al. Toxicology in Vitro, 2013，27(2)：954. [12] Page S E, Arnold W A, McNeill K. Journal of Environmental Monitoring, 2010, 12(9): 1658. [13] Xiang Q, Yu J, Wong P K. J. Colloid. Interface. Sci., 2011, 357(1): 163. [14] Biondi R, Xia Y, Rossi R, et al. Anal. Biochem., 2001, 290(1): 138. [15] Vera-Jimenez N I, Pietretti D, Wiegertjes G F, et al. Fish Shellfish Immunol., 2013, 34(5): 1216.
