Detection of Antioxidative Capacity of Bamboo Leaf Extract by Scavenging Superoxide Anion Free Radical
GUO Xue-feng,YUE Yong-de*,TANG Feng,WANG Jin,YAO Xi
International Centre for Bamboo and Ratten, State Forestry Administration Key Open Laboratory on Bamboo and Rattan Science and Technology, Beijing 100102, China
Abstract：By studying absorption spectrum, oxidation velocity of pyrogallic acid reaction system, the effect of pyrogallic acid concentration and buffer pH value on the scavenging rate for superoxide anion free radical coming from reaction system was investigated, and some results were obtained as follows, the wavelength used for the pyrogallic acid reaction system was determined by spectrophotometric method：319.5 nm.Some parameters of the reaction system were determined as follows：reaction volume：10 mL, reaction time：9 min, volume of pyrogallic acid(3 mmol·L-1)：0.3 mL, pH value of buffer：8.2 and oxidation velocity：0.035.The relation between scavenging rate for superoxide anion free radical and concentration of TBHQ and bamboo leaf extract samples was studied by our method, and IC50 (the value of antioxidant concentration at scavenging half of superoxide anion free radical) was used as the index to evaluate scavenging capacity.IC50 value of TBHQ and most effective samples were as follows：TBHQ(95.01 mg·L-1) and M40(298.69 mg·L-1).M40 and other bamboo leaf extract could be used as natural antioxidant.
Key words：Bamboo Leaf Extract;Superoxide anion free radical.;Scavenging rate;IC50 value
[1] Osawa T, Namiki M A.Agric.Biological Chem., 1981, 45(3)：735. [2] Ottolenghi A.Arch.Biochem.Biophys, 1959, 79：355. [3] Cao G H, Alessio H M, Culter R G.Free Radical Biol.Med., 1993, 14(3)：303. [4] WU Yong-jun, SHI Jie, QU Ling-bo, et al(吴拥军, 石 杰, 屈凌波, 等).Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(9)：1688. [5] GUO Xue-feng, YUE Yong-de, TANG Feng, et al(郭雪峰，岳永德，汤 锋, 等).Spectroscopy and Spectral Analysis(光谱学与光谱分析), 待发表. [6] SONG Huai-en，WEN Ren(宋怀恩, 闻 韧).Chinese Journal of Medicinal Chemistry(中国药物化学杂志), 2003, 13(2)：119. [7] ZHENG Jing-quan(郑晶泉).Foreign Medical Sciences(Section of Hygiene)(国外医学, 卫生学分册), 2000, 27(1)：37. [8] LIANG Ai-hui, JIANG Zhi-liang, ZHOU Su-mei, et al(梁爱惠, 蒋治良, 周苏梅, 等).Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(11)：2113. [9] JIANG Liu-yun, LIU Yu-ming(蒋柳云, 刘玉明).Chinese Journal of Organic Chemistry(有机化学), 2005, 25(6)：684. [10] FAN Zhi, ZHAO Ru, ZHOU Wei-hong, et al(樊 志, 赵 茹, 周卫红, 等).Journal of Tianjin Normal University(Natural Science Edition)(天津师范大学学报·自然科学版), 1999, 19(3)：16. [11] LI Ping, WANG Yan-hui, MA Run-yu(李 平, 王艳辉, 马润宇).Journal of Beijing University of Chemical Technology(北京化工大学学报), 2003, 30(3)：35. [12] LIN Gui-lan, XU Xue-shu, LIAN Wen-si(林桂兰, 许学书, 连文思).Journal of East China University of Science and Technology(Natural Science Edition)(华东理工大学学报·自然科学版), 2006, 32(3):278. [13] ZHANG Li-wei，WANG Jin-shan，JIANG Chong-qiu(张立伟, 王金山, 江崇球).Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(9)：1103. [14] LU Bo-yi, ZHANG Ying, WU Xiao-qin(陆柏益, 张 英, 吴晓琴).Chemistry and Industry of Forest Products(林产化学与工业), 2005, 25(3)：120. [15] ZHANG Xi-qin(张希琴).Journal of Qingdao University(Engineering & Technology Edition)(青岛大学学报·工程技术版), 2001, 16(2)：6.
