Determination of the Contents of Chlorogenic Acid and Phillyrin of Shuanghuanglian Oral Fluid Using NIRS
DAI Chuan-yun1, GAO Xiao-yan2，TANG Bo1, FU Ya1, LIU Huo-an1
1. Department of Biology, Chongqing University of Science and Technology, Chongqing 401331, China 2. Science Development Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
Abstract：The aim of the present study was to establish the model of predicting the contents of chlorogenic acid and phillyrin in Shuanghuanglian oral fluid using NIR to realize quick quality evaluation of Shuanghuanglian oral fluid. To this end, many batches of Shuanghuanglian oral fluid were selected, and the contents of chlorogenic acid and phillyrin were determined using HPLC. Meanwhile, the NIR spectra of the same samples were determined. The model used to predict the contents of chlorogenic acid and phillyrin in Shuanghuanglian oral fluid was established by correlation analysis between the results gained by HPLC and NIR spectra. According to the value of RSEP and r, the method of data processing was chosen. The method of spectra processing and wavelength range or wave numbers were chosen based on the value of RMSECV. The method of data processing was SMLR. The original spectra were used to establish the model. The wave numbers in the model used to predict the contents of chlorogenic acid and phillyrin were 6 654.06/7 106.08 cm-1, and 5 456.06/7 222.08 cm-1 respectively. The RMSECV and the correlation coefficient of the best model of chlorogenic acid and phillyrin were 0.857 26, 0.889 87 and 0.857 26 and 0.889 87. The results of cross validation indicate that the predicting model was accurate and credible, and could be used as a rapid quality control method of Shuanghuanglian oral fluid.
戴传云1，高晓燕2，汤 波1，傅 亚1，刘火安1 . 近红外光谱法测定双黄连口服液中绿原酸和连翘苷的含量[J]. 光谱学与光谱分析, 2010, 30(02): 358-362.
DAI Chuan-yun1, GAO Xiao-yan2，TANG Bo1, FU Ya1, LIU Huo-an1 . Determination of the Contents of Chlorogenic Acid and Phillyrin of Shuanghuanglian Oral Fluid Using NIRS . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(02): 358-362.
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