Investigation of Typical Melamine Urinary Stones Using Infrared Spectra
SI Min-zhen1,2, LI Qing-yun3, LIU Ren-ming2, KANG Yi-pu2, WANG Kun-hua4, ZHANG Zhi-guo1
1. Department of Applied Physics, Harbin University of Technology, Harbin 150080, China 2. Department of Physics and Electronic Science, Chuxiong Normal Univesity, Chuxiong 675000, China 3. Foundamental Department of South West Forest College, Kunming 650224, China 4. First People’s Hospital of Yunnan, Kunming 650032, China
Abstract：A typical melamine kidney stone confirmed by some medicine expert was collected from the first people’s hospital of Yunnan. The kidney stone was adequately determined by PE corporation spectra 100(with resolution of 1 cm-1). The stone samples for FTIR analysis were prepared using the KBr pellet technique, where 2 mg of the pretreated stone powder was mixed with 200 mg of analytical grade KBr using an agate pestle and mortar. The digital spectrum was then scanned in the mid-infrared region from 4 000 to 400 cm-1 at room temperature. The appearing bands between 4 000 and 2 000 cm-1 were 3 487，3 325，3 162 and 2 788 cm-1，those between 1 700 and 1 000 cm-1 were 1 694，1 555，1 383，1 340，1 189 and 1 122 cm-1，and those between 1 000 and 400 cm-1 were 993，782，748，709，624，585，565 and 476 cm-1. It was found that the main constituent of calculi showed few comparability with cat kidney stone, which was from cats that died after consuming the contaminated food, and confirmed that these deposits were primarily composed of melamine and cyanuric acid compared to the IR spectra of calculi in literature.It was also found that the main constituent of calculi showed few comparability with popular kidney stone by comparison with the IR spectra of calculi in literature. The spectrum of calculi was 50% respectively similar with melamine and uric acid as compared with the IR spectrum. It was found that the main constituent of calculi was melamine itself and uric acid as compared with the IR spectra of calculi and melamine: (1∶1), because the spectrum of calculi was 83.3% similar to melamine and uric acid (1∶1). The appearing bands of melamine and uric acid (1∶1) between 4 000 and 2 000 cm-1 were 3 469，3 419，3 333，3 132，3 026，2 827 cm-1，those between 1 700 and 1 000 cm-1 were 1 696，1 656，1 555，1 489，1 439，1 350，1 311，1 198，1 124 and 1 028 cm-1，and those between 1 000 and 400 cm-1 were 993，878，814，784，745，708，619，577 and 475 cm-1.
司民真1,2, 李清玉3, 刘仁明2, 康颐璞2, 王昆华4，张治国1 . 典型的三聚氰胺致幼儿肾结石红外光谱分析[J]. 光谱学与光谱分析, 2010, 30(02): 363-367.
SI Min-zhen1,2, LI Qing-yun3, LIU Ren-ming2, KANG Yi-pu2, WANG Kun-hua4, ZHANG Zhi-guo1 . Investigation of Typical Melamine Urinary Stones Using Infrared Spectra. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(02): 363-367.
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