Study on the Synergistic Antioxidant Mechanism of Chlorogenic Acids (CQAs) with Electrochemical and Spectroscopy Property
YANG Kai-zhou1,3, ZHAI Xiao-na1, WANG Jia-liang1, CHAI Zhi1, REN Fa-zheng1, LENG Xiao-jing1,2*
1. Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China 2. Beijing Innovation Team of Modern Agricultural Industry Technology System, Beijing 100083, China 3. Beijing Zhugula Coffee Co., Ltd., Beijing 100085, China
Abstract：The synergistic antioxidant mechanism of chlorogenic acids (CQAs) was studied in this paper through cyclic voltammograms (CV), oil-water partition coefficient (P), FT-IR, XRD and circular dichroism (CD). The antioxidant capability of CQAs isomers and their mixture was determined by using ABTS free radical quenching ability assay. The results showed that the bigger the antioxidant activity disparity between the CQAs molecules was, the higher the content of high antioxidant activity CQAs was, the better the synergistic effect of the CQAs combination mixture became; The oxidation potential (Epa) of CQAs combination mixture kept constant in the synergistic experiments, which indicted the oxidative coupling interaction don’t exist between the CQAs; The charge transferred (Q) and antioxidant activity exhibited high correlation (0.92); the practical Q was higher than the theoretical Q in the synergistic process and this confirmed that the CQAs (dicaffeoylquinic acids) regeneration of high antioxidant activity happened; the CQAs mixture with the absolute difference value of oil-water partition coefficient of 0.13 gave the good interface effect and high synergistic degree; the interaction and the regular arrangement between the CQAs combination were not discovered through FT-IR, XRD and CD. Therefore, the regeneration mechanism of CQAs molecules and the interface effect of reaction system were the main cause of the phenomenon of the synergistic antioxidant of CQAs.
杨剀舟1，3，翟晓娜1，王佳良1，柴 智1，任发政1，冷小京1，2* . 绿原酸协同抗氧化机理的电化学和光谱-色谱学研究 [J]. 光谱学与光谱分析, 2016, 36(08): 2405-2413.
YANG Kai-zhou1,3, ZHAI Xiao-na1, WANG Jia-liang1, CHAI Zhi1, REN Fa-zheng1, LENG Xiao-jing1,2* . Study on the Synergistic Antioxidant Mechanism of Chlorogenic Acids (CQAs) with Electrochemical and Spectroscopy Property . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(08): 2405-2413.
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