Study on the Identification of Six Kinds of Bee Pollens by Three-Step Infrared Macro-Fingerprint Method
WU Jie1, 3, ZHOU Qun2, WU Li-ming1，AN Jian-dong1, SUN Su-qin2*, HU Fu-liang3
1. Institute of Apicultural Research, the Chinese Academy of Agricultural Sciences, Beijing 100093, China 2. Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China 3. College of Animal Science, Zhejiang University, Hangzhou 310029, China
Abstract：Six kinds of bee pollens, including apricot pollen, lotus pollen, rape pollen, camellia pollen, watermelon pollen and corn poppy pollen, were identified non-destructively by Fourier transform infrared spectroscopy (FTIR) combined with derivative spectra and two-dimensional correlation spectroscopy (2D) in the present article. Compared with conventional IR spectra of samples, some certain differences were found in the characteristic peaks of proteins, lipids and carbohydrates. Obvious differences of the six kinds of bee pollens were found in the second derivative spectra. And in the 2D-IR correlation spectra, the samples presented the differences in the position and intensity of the autopeaks and correlation peak clusters. Therefore, the three-step IR macro-fingerprint provides a more rapid and effective method for the identification of different kinds of bee pollens.
吴 杰1, 3，周 群2，吴黎明1，安建东1，孙素琴2*，胡福良3 . 六种蜂花粉的红外光谱三级鉴别研究[J]. 光谱学与光谱分析, 2010, 30(02): 353-357.
WU Jie1, 3, ZHOU Qun2, WU Li-ming1，AN Jian-dong1, SUN Su-qin2*, HU Fu-liang3 . Study on the Identification of Six Kinds of Bee Pollens by Three-Step Infrared Macro-Fingerprint Method. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2010, 30(02): 353-357.
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