光谱学与光谱分析
桑色素-Ce(Ⅳ)体系共振光散射法选择性测定小牛胸腺DNA
周敏，马永钧，张德懿，陈慧*
西北师范大学化学化工学院，甘肃 兰州 730070
Selective Determination for Calf Thymus DNA by a Resonance Light Scattering Technique with Morin-Ce(Ⅳ) System
ZHOU Min, MA Yong-jun, ZHANG De-yi, CHEN Hui*
Institute of Chemistry, Northwest Normal University, Lanzhou 730070, China
摘要 ： 研究了桑色素与Ce(Ⅳ)反应形成的络合物与DNA作用的共振光散射(RLS)特征，发现小牛胸腺DNA(ctDNA)或鲱鱼精DNA(hsDNA)的加入都能使桑色素-Ce(Ⅳ)体系在320 nm处的共振光散射峰增强。在最优条件下，RLS强度与ctDNA的浓度在0～25 μg·mL-1 范围内呈良好的线性关系，检出限为0.3 μg·mL-1 ;但是RLS强度对hsDNA的浓度却没有线性响应。并且，由于RLS强度对hsDNA浓度的响应值大大低于同浓度的ctDNA，因此可用于hsDNA存在下对ctDNA的选择性测定。将该法用于4种合成样的测定，回收率在93.7%～108.4%之间。
关键词 ：桑色素;DNA;共振光散射
Abstract ：The interaction of DNA and the binary complex formed between Ce(Ⅳ) and morin was studied. With the maximum scattering peak located at 320 nm, the enhanced RLS was proportional to the concentration of ctDNA in the range 0-25 μg·mL-1 and the detection limit (3σ ) of 0.3 μg·mL-1 was obtained. Under the optimized conditions, four synthetic samples were determined with recoveries in the range 93.7%-108.4%. The proposed method is particularly attractive for its high selectivity for ctDNA in the presence of hsDNA by using certain sensitive spectrofluorometer since the intensity of RLS for hsDNA is far weaker than that for ctDNA.
Key words ：Morin;DNA;Resonance light scattering
收稿日期: 2005-12-06
修订日期: 2006-05-18
通讯作者:
陈慧
引用本文:
周敏，马永钧，张德懿，陈慧* . 桑色素-Ce(Ⅳ)体系共振光散射法选择性测定小牛胸腺DNA[J]. 光谱学与光谱分析, 2006, 26(09): 1656-1659.
* . Selective Determination for Calf Thymus DNA by a Resonance Light Scattering Technique with Morin-Ce(Ⅳ) System. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(09): 1656-1659.
链接本文:
https://www.gpxygpfx.com/CN/Y2006/V26/I09/1656
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