Analysis of Belamcanda Chinensis with Space Flight Mutagenesis by FTIR
ZHANG Hong-mei1, GUAN Ying1, SHI Jin-shan2
1. College of Science, Yanshan University, Qinhuangdao 066004, China 2. Institute of Electrical Engineering, Yanshan University, Qinhuangdao 066004, China
Abstract:The amorphous active components of the space mutagenesis Belamcanda chinensis and the ground group were measured, compared and analyzed. The purpose was to get a comprehensive understanding of the changes in quality of the 4th generation space mutagenesis Belamcanda chinensis, accumulate data for further studies, and try to establish the quality criterions of space mutagenesis Belamcanda chinensis. The result shows that the FTIR spectra of the space sample are almost the same as that of the ground one in terms of the main absorption peaks positions and shapes, but there are obvious differences in intensities. The intensity of the absorption peak at 1 642 and 1 318 cm-1 of the space group was remarkably enhanced than the ground group, but at 1 541, 1 456, 1 417 and 1 051 cm-1 it was decreased compared to the ground group. At the same time, the peak at 1 642 cm-1 of the stretching vibration of CO, the characteristic absorption of the keto, was remarkably enhanced. The peaks at 1 541 and 1 456 cm-1 were assigned to C—C groups, the peak at 1 417 cm-1 was due to the —CH2— groups, the peak at 1 318 cm-1 was the characteristic absorption of calcium oxalate monohydrate, and the peak at 1 051 cm-1 was assigned to C—O groups. It was shown that the relative content of flavone was increased distinctly. Space mutation breeding is conducive to breeding new varieties of highly active ingredients, it is also one of the ways to innovate germplasm resources of Chinese medicines efficiently. The effect of the space group is expected to be enhanced than the ground group, but it needs to be proved through further research .
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