Qualitative and Quantitative Analysis of Shuanghuanglian Fenzhenji by FTIR
YAN Shu1, XU Mao-ling1, TU Ya2, LI Hui-fen3*, SUN Su-qin2
1. Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China 2. Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China 3. Tianjin Medical University，Tianjin 300070, China
Abstract：Qualitative and quantitative analysis of Shuanghuanglian fenzhenji was studied in the present paper by FTIR. The fingerprint characteristics of main components in the injections were assigned and can be used as the criterion in the qualitative quality control. Calibration models by PLS algorithm were built to get the quantitative information of baicalin and chlorogenic acid in the injection samples. The R2 of calibration models are both above 0.99 and the average relative deviations between predicted and actual value measured by HPLC are less than 4% for the both properties. The qualitative and quantitative analysis by FTIR is simple and rapid, so it may provide a new on-line approach to the quality control of main constituent in Shuanghuanglian fenzhenji and some other traditional Chinese medicines.
Key words：Shuanghuanglian fenzhenji;FTIR;Qualitative and quantitative analysis
阎姝1, 徐茂玲1, 图雅2, 李惠芬3*, 孙素琴2 . 红外光谱法对双黄连粉针剂的定性定量分析[J]. 光谱学与光谱分析, 2009, 29(06): 1558-1561.
YAN Shu1, XU Mao-ling1, TU Ya2, LI Hui-fen3*, SUN Su-qin2 . Qualitative and Quantitative Analysis of Shuanghuanglian Fenzhenji by FTIR. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(06): 1558-1561.
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