Analysis of the 4th Generation Scutellaria Baicalensis Georgi with Space Mutagenesis Via FTIR Spectroscopy
DING Xi-feng1,GAO Hua-na1,GUO Xi-hua1,WANG Zhi-zhou1,YANG La-hu2, GUAN Ying1*, SHI Jin-shan3
1. College of Sciences,Yanshan University,Qinhuangdao 066004,China 2. National Institute for Control of Pharmaceutical and Biological Product, Beijing 100050, China 3. Institute of Electrical Engineering, Yanshan University, Qinhuangdao 066004, China
Abstract:Determination and analysis for the Scutellaria baicalensis Georgi with space mutagenesis and the ground group were carried out with FTIR for the first time in order to fully understand the quality changes of the 4th generation of space mutagenesis of Scutellaria baicalensis Georgi and do further research. The result shows that for the FTIR spectra of the two samples the position and shape of main absorption peaks are approximately the same, indicating that the major components and the structures of space group remain intact, but the intensities of the absorption peaks are obviously different, with the intensities of the space group significantly enhanced compared to the ground group, especially for the flavonoid compounds (baicalin, baicalein, wogonoside, wogonin and wogonoside etc), which are the main active components of the Scutellaria baicalensis Georgi, the absorption peak intensities at 3 391, 1 655 and 1 069 cm-1 are obviously stronger than those of the ground group, so the contents of flavonoid compounds and other components are higher, and the amorphous active components are optimized. Space mutation breeding is conducive to breeding new varieties of highly active components, and it is also one of the ways to innovate Chinese medicines germplasm resources efficiently.
[1] GUAN Ying, DING Xi-feng, WANG Wen-jing, et al(关 颖,丁喜峰,王文静,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2008,28(2):460. [2] GUO Xi-hua, GUAN Ying, DI Li-jie, et al(郭西华,关 颖,邸立杰,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2008,28(6): 1283. [3] GUAN Ying, YANG La-hu, DING Xi-feng, et al(关 颖,杨腊虎,丁喜峰,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2008,28(5): 1191. [4] WANG Wen-jing, GUAN Ying, SUN Xin, et al(王文静,关 颖,孙 鑫,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2008,28(12): 2993. [5] PEI Li-kuan,GUO Bao-lin,SUN Su-qin,et al(裴利宽,郭宝林,孙素琴,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2008,28(1):55. [6] ZHOU Xin,SUN Su-qin,HUANG Qing-hua(周 欣,孙素琴,黄庆华). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2007,27(12):2453. [7] ZUO Kang, LI Dan-ting, GUO Shui-liang, et al(左 康,李丹婷,郭水良,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2007,27(10):1989. [8] CHEN Jun, ZHOU Qun, YU Lu, et al(陈 军,周 群,郁 露,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2007,27(9): 1723. [9] CHEN Jian-bo, ZHOU Qun, SUN Su-qin, et al(陈建波,周 群,孙素琴,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2007,27(8): 1493. [10] WU Jing,SUN Su-qin,ZHOU Qun,et al(吴 婧,孙素琴,周 群,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2007,27(8):1535. [11] ZHANG Peng, SUN Su-qin, ZHOU Qun, et al(张 鹏, 孙素琴, 周 群, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2007,27(8):1539. [12] XU Yong-qun,SUN Su-qin,FENG Xue-feng, et al(徐永群,孙素琴,冯学峰,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2003,23(3):502. [13] LIU Dai, MAO Hong-de, YANG Li-xin, et al(刘 岱,毛宏德,杨立新,等). Lishizhen Medicine and Materia Medica Research(时珍国医国药),2001,12(10):934. [14] WANG Min,SUN Zeng-xian(王 敏,孙增先). Journal of Huaihai Medicine(淮海医药),2007,25(5):484. [15] CHI Ge-fu,DING Li,CHANG Li-min(迟戈夫,丁 丽,常丽敏). Journal of Inner Mongolia University for Nationalities(内蒙古民族大学学报·自然科学版),2005,20(2):207. [16] LI Yun-xia, SUO Quan-ling, HE Wen-zhi, et al(李云霞,索全伶,贺文智,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2007,27(1):131. [17] HU Shi-lin,FENG Xue-feng(胡世林,冯学锋). Chinese Pharmaceutical Journal(中国药学杂志),2001,36(11):728.