Determination the Chemical Speciation of Cu，Zn，Fe and Mn in Radix Scutellariae by AAS
MIAO Shan1, SUN Ji-yuan1, XIE Yan-hua1, WANG Jian-bo1, SHI Xiao-peng2, DING Yuan-yuan1, BI Lin-lin1, GAO Shuang-bin2, WANG Si-wang1*
1. Institute of Materia Medica，School of Pharmacy, Fourth Military Medical University, Xi’an 710032 China 2. Department of Pharmacy of Xijing Hospital，Fourth Military Medical University，Xi’an 710032 China
Abstract：An analysis method was developed to determine the chemical speciation of Cu, Zn, Fe and Mn in radix scutellariae decoction using atomic absorption spectroscopy(AAS). The decoction can be divided into suspension and soluble species by 0.45 μm filter membrane and the soluble species can be separated into organism and inorganic species by LSA-10 macroporous resin. These elements in water-soluble test samples can be divided into alcohol-soluble and water-soluble by adopting n-octyl alcohol-water allocation system in man-made gastric acidity. Then, the concentration of these elements was determined by AAS, which provided more chemical speciation information about these elements instead of the total amount of them only in radix scutellariae. Detection limit of Cu, Zn and Mn by using the method was all 0.01 μg·mL-1 and was 0.02 μg·mL-1 for Fe. The RSD was in the range of 1.5％-3.6％(n=11) and the recovery rate of soluble species and inorganic species were in range of 96.7％-105.0％. The method has been successfully applied to determine the chemical speciation of Cu, Zn, Fe and Mn in radix scutellariae, which was very important for overall study of radix scutellariae.
缪珊1,孙纪元1,谢艳华1,王剑波1,石小鹏2,丁媛媛1,毕琳琳1,高双斌2,王四旺1* . 原子吸收法测定中药黄芩中不同化学形态的Cu、Zn、Fe和Mn[J]. 光谱学与光谱分析, 2009, 29(05): 1427-1430.
MIAO Shan1, SUN Ji-yuan1, XIE Yan-hua1, WANG Jian-bo1, SHI Xiao-peng2, DING Yuan-yuan1, BI Lin-lin1, GAO Shuang-bin2, WANG Si-wang1*. Determination the Chemical Speciation of Cu，Zn，Fe and Mn in Radix Scutellariae by AAS. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(05): 1427-1430.
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