A Study on Decreasing the Instrument Detection Limit of Atomic Fluorescence Spectrometry(AFS-930)for Hg
YIN Xue-bin1, LU Xiao-qi1, YAO Chun-xia1, SONG Jing1, QIAN Wei1, LUO Yong-ming1*, LIANG You-qing2, SUN Li-guang2
1. Soil and Environment Bioremediation Research Center, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China 2. Institute of Polar Environment, University of Science & Technology of China, Hefei 230026, China
Abstract：In the present study, the detection limit of atomic fluorescence spectrometry (AFS-930) was decreased to 2 ng·L-1 (n=6) based on several optimizing modifications, including that the sub-high voltage of photomultiplier tube and the current of hollow-cathode lamp were elevated to 280 V and to 30 mA, respectively, and the height of atomization cell was set as 10 mm; In addition, the concentration of KBH4 was decreased to 0.5% (KOH 0.2%). With the optimized parameters, a good standard curve of Hg concentration versus intensity of fluorescence (If) could been obtained readily, after that, a 4-ng·L-1-Hg water samples was measured accurately with a little relative standard deviation (RSD) of <5%, while for approximately 2-ng·L-1-Hg waters the RSD varied within a wide range of 10.9%-27.2%, likely due to the absorption of Hg by polyethylene vessels used in this study and/or due to the contamination by analysis grade reagents used in this study. By using low-absorption polytetrafluoroethylene (PTFE) materials and the guaranteed reagents, the instrument detection limit was further decreased to 1 ng·L-1(n=10).
尹雪斌1,陆晓奇1,姚春霞1,宋静1,钱薇1,骆永明1*,梁有庆2,孙立广2 . 关于降低AFS-930型原子荧光光度计汞检出限的方法研究[J]. 光谱学与光谱分析, 2009, 29(05): 1431-1433.
YIN Xue-bin1, LU Xiao-qi1, YAO Chun-xia1, SONG Jing1, QIAN Wei1, LUO Yong-ming1*, LIANG You-qing2, SUN Li-guang2. A Study on Decreasing the Instrument Detection Limit of Atomic Fluorescence Spectrometry(AFS-930)for Hg. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(05): 1431-1433.
[1] GUO Xiao-wei, GUO Xu-ming(郭小伟, 郭旭明). Chinese Journal of Analysis Laboratory(分析试验室), 1993, 12(4): 92. [2] MEI Jun, WANG Xiu-ji, JIN Ze-xiang, et al(梅 俊, 王秀季, 金泽祥, 等). Chinese Journal of Analysis Laboratory(分析试验室), 2004, 23(3): 31. [3] Beijing Qitan Co. (北京吉天仪器有限公司). Manual of AFS (原子荧光分析方法手册). 2005. [4] SUO You-rui, HUANG Ya-li(索有瑞, 黄雅丽). Chinese Journal of Spectroscopy Laboratory(光谱实验室), 1991, 8(4): 10. [5] YAO Chun-xia, YIN Xue-bin, ZHANG Chang-bo, et al(姚春霞, 尹雪斌, 张长波, 等). Soil(土壤), 2006, 38(5): 534. [6] Sun L G, Yin X B, Liu X D, et al. Sci. Total. Environ., 2006, 368: 236. [7] YIN Xue-bin, YAO Chun-xia, LUO Yong-ming, et al(尹雪斌, 姚春霞, 骆永明, 等). Chinese Journal of Analysis Laboratory(分析试验室), 2006, 25(10): 119. [8] Temminghoff E J M, Plette A C C , Van Eck R, et al . Anal. Chim. Acta, 2000, 417: 149. [9] Weng L P, Lexmond T M, Wolthoorn A, et al. Environ. Toxicol. Chem., 2003, 22: 2180. [10] Kalis E J J, Weng L P, Dousma F, et al. Environ. Sci. Technol., 2006, 40: 955. [11] Sigg L, Black F, Buffle J, et al. Environ. Sci. Technol., 2006, 40: 1934. [12] Docekalova H, Divis P. Talanta, 2005, 65: 1174. [13] Clarisse O, Hintelmann H. J. Environmelal Monitoring, 2006, 8: 1242.
