Study on Measurement of Mercury Ion in Water by Thiamine-Fluorescence Excitation-Emission Matrix
JULDEZ Nurlan1，2，3, SHEN Jian1，2，3, LENG Xiao-ting3, CHAI Yi-di1，2，3, WANG Shi-feng3, HU Yuan3, CUI Hao-yue3, WU Jing1，2，3*
1. State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China
2. Research Center of Environmental Technology in Pollution Source Identification and Precise Supervision, School of Environment, Tsinghua University, Beijing 100084, China
3. Research and Development Center of Advanced Environmental Supervision Technology and Instrument, Research Institute for Environmental Innovation (Suzhou), Tsinghua, Suzhou 215163, China
Abstract：Mercury is a heavy metal element with significant accumulation effect and genotoxicity, which is extremely harmful to human health and the ecological environment. In China, the water environment is facing serious problems of mercury pollution. Developing rapid, efficient, and economical method for mercury ion detection can effectively promote the source control of mercury pollution in the aquatic environment. This study innovatively proposed a method, namely thiamine-fluorescence excitation-emission matrix (EEM), to monitor mercury ion in the water environment. Results showed that the position and number of fluorescence peaks of thiamine significantly changed after it happed redox reaction with mercury ion, which could be used as a characteristic signal for detecting mercury ions in water. In addition, when using this method to detect mercury ions in water, it was suggested that the concentration of thiamine should not be too high, and the reaction system should be kept in the alkaline environment. The reaction temperature and reaction time could be further optimized by the first-order kinetic model to reduce the detection cost and improve the detection effectiveness. Under the specific detection conditions (thiamine concentration 10 μmol·L-1, pH 9.7, reaction time 120 min, temperature 20 ℃)，the linear detection range of mercury ion concentration was suggested to 4～15 μmol·L-1. The thiamine-EEM method owns outstanding advantages and good practical application values compared with the traditional method of mercury ion monitoring in water, which can effectively help the pollution source supervision of mercury in water environment and greatly improve the efficiency of environmental law enforcement.
祖丽德孜·努兰，沈 鉴，冷晓婷，柴一荻，王士峰，胡 远，崔皓月，吴 静. 硫胺素-三维荧光法测定水中汞离子的研究[J]. 光谱学与光谱分析, 2021, 41(06): 1846-1851.
JULDEZ Nurlan, SHEN Jian, LENG Xiao-ting, CHAI Yi-di, WANG Shi-feng, HU Yuan, CUI Hao-yue, WU Jing. Study on Measurement of Mercury Ion in Water by Thiamine-Fluorescence Excitation-Emission Matrix. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(06): 1846-1851.
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