Thermochemical Studies on the Binding Reaction of Perseleno Diphenyl 2,2’-Diformic Acid with Bovine Serum Albumin
YAN Cheng-nong1，SHANGGUAN Yun-feng1，LIU Yi2, HOU An-xin2, QU Song-sheng2
1. College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou 434020, China 2. College of Chemistry and Molecule Sciences, Wuhan University, Wuhan 430072, China
Abstract：The binding of newly compounded perseleno diphenyl 2,2’-diformic acid to bovine serum albumin(BSA) was studied at different temperatures using fluorescence spectrum and UV spectrum. The fluorescence quenching data was analyzed according to Stern-Volmer equation and Lineweaver-Burk double-reciprocal equation. It was showed that this quenching complies better with the charactristic of static fluorescence quenching. The binding constant, thermodynamic parameters, and the binding spot of the compound with certain structure, coming from perseleno diphenyl 2,2’-diformic acid and bovine serum albumin, were obtained. Besides, the mechanism of static fluorescence quenching and the quality of binding power were both discussed. The information of the binding mode, the mechanism of its transportation, and some medical theories in human body were offered.
